PHARMACOLOGICAL STUDY OF PROTECTIVE EFFECT OF METHYL PALMITATE IN EXPERIMENTALLY-INDUCED MYOCARDIAL INFARCTION
Ahmed Badreldin Abdelaal Hamed;
Abstract
The present study aimed to investigate the potential cardioprotective effect of methyl palmitate (MP) against isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Moreover, the study aimed to elucidate the underlying molecular mechanisms via studying its effect on different oxidative stress, apoptotic and inflammatory markers. The study was carried out in two consequent phases:
Phase I: Assessment of the cardioprotective dose of MP
Fifty male Sprague Dawley rats were randomized into five groups (n=10). Group 1 was considered as a control group, where rats were given corn oil, orally, three times per week on alternative days for 2 weeks. Rats in group 2 were given corn oil, orally, three times per week on alternative days for 2 weeks and then injected with 2 consecutive doses of ISO (85 mg/kg, s.c) on the 13th and 14th days. Rats in groups 3, 4 and 5 were given different doses of MP (75, 150 and 300 mg/kg, respectively, p.o) three times per week on alternative days, then injected with 2 consecutive doses of ISO (85 mg/kg s.c.) on the 13th and 14th days. After 24 hours from the last ISO injection, serum samples were collected from each group, then the rats were sacrificed and heart tissues were isolated.
Studied parameters in phase I
Histopathological examination of heart tissues using Hematoxylin and Eosin (H & E) stain was performed. Serum activities of the cardiac enzymes creatine kinase myocardial band (CK-MB), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were detected as well. Additionally, heart index was calculated.
Phase I: Assessment of the cardioprotective dose of MP
Fifty male Sprague Dawley rats were randomized into five groups (n=10). Group 1 was considered as a control group, where rats were given corn oil, orally, three times per week on alternative days for 2 weeks. Rats in group 2 were given corn oil, orally, three times per week on alternative days for 2 weeks and then injected with 2 consecutive doses of ISO (85 mg/kg, s.c) on the 13th and 14th days. Rats in groups 3, 4 and 5 were given different doses of MP (75, 150 and 300 mg/kg, respectively, p.o) three times per week on alternative days, then injected with 2 consecutive doses of ISO (85 mg/kg s.c.) on the 13th and 14th days. After 24 hours from the last ISO injection, serum samples were collected from each group, then the rats were sacrificed and heart tissues were isolated.
Studied parameters in phase I
Histopathological examination of heart tissues using Hematoxylin and Eosin (H & E) stain was performed. Serum activities of the cardiac enzymes creatine kinase myocardial band (CK-MB), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were detected as well. Additionally, heart index was calculated.
Other data
| Title | PHARMACOLOGICAL STUDY OF PROTECTIVE EFFECT OF METHYL PALMITATE IN EXPERIMENTALLY-INDUCED MYOCARDIAL INFARCTION | Other Titles | دراسة فارماكولوجية للتأثير الوقائي الخاص بميثيل بالميتات في إحتشاء عضلة القلب الناجم تجريبياً | Authors | Ahmed Badreldin Abdelaal Hamed | Issue Date | 2020 |
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