EFFECT OF PROBIOTIC SUPPLEMENTATION TO FATTENING ANIMAL RATION ON ITS PRODUCTIVE PERFORMANCE

Abstract

The probiotic bacteria used in this study is a mixture of 15 isolate of lactobacillus sp (Eleven probiotic bacteria isolates were isolated from calves saliva and feces plus 4 isolates were isolated from rumen). The isolates were characterized and identified then propagated to use it as a probiotic supplementation. This work was a part of "production of probiotic bacteria and evaluation its impact on dairy and beef cattle" funded by STDF (ID: 10802). Lactobacilli isolates were grown on MRS broth (Oxoid) and Streptococci isolates were grown on M17 broth (Difco), after that the broth media incubated for 24 h at 37 °C. The strains were activated two or three times in order to obtain high biomasses in the stationary phase. The present study was divided into two experiments, the first was in vitro experiment and it was aimed to evaluate effect of different level of probiotic supplementation to ruminant rations, using in-vitro batch culture technique to determine degradation and fermentation parameters. The second study was Fattening study this study was to evaluate effect of supplementing ruminant ration with probiotic bacteria supplementation on its productive performance. The best level of probiotic supplementation in the in vitro experiment was subjected for further in vivo investigation. The fattening study divided into to trials, 1st fattening trial and 2nd fattening trial; 1- In vitro experiment In vitro experimental ration was formulated, the ration consisted of 40% alfalfa hay and 60% concentrate feed mixture. Three level of probiotic supplementation (106, 108, 1010 cfu/kg DM) were evaluated. DM and total gas production as well as fermentation parameters of the incubated samples were determined after 24 hrs. of fermentation.