BIOLOGICAL SYNTHESIS OF COPPER NANOPARTICLES USING BACTERIA AND ITS APPLICATION AS ANTIMICROBIAL AND ANTITUMOR AGENTS

Abstract

Biological synthesis of nanoparticles is a recent trend that has become a domain of research as an alternative to traditional methods, whether chemical or physical, which are high-cost and have residues with extreme side effects that are difficult to overcome. Bacteria are one of the most important new methods for the safe and rapid biosynthesis of nanoparticles. It advantages from their rapid growth, the ease of controlling all conditions (nutritional and environmental) and simple nutritional needs, as well as the improvement of the properties and quality of biosynthesized nanoparticles. Many researches interested in the biosynthesis of copper metal nanoparticles which is characterized by low-cost and availability in large quantities compared to other metal nanoparticles such as gold, silver, and platinum. The objectives for this thesis concerned with isolation, screening, selection and identification of the most potent CuNPs synthesizing bacterial strain. In addition to exploitation of cheap agricultural wastes and by-products as sources of carbon and nitrogen nutrition in order to improve the production process and reduce the costs used for production. Then, optimization of CuNPs production by investigating the interaction between all the affecting factors. Improving the CuNPs properties was aimed to increase the stability and biocompatibility with biological applications and studies of antimicrobial activity and antitumor.