Immunological Studies on Salmonellae Isolated From Different Sources

Abstract

Salmonellosis is one of the major global health concerns leading to millions of deaths annually. Accurate and fast detection of harmful Salmonella is a major concern of food safety. Serology is commonly used to monitor disease in poultry, therefore application of Salmonella serotype-specific test will have added value in Salmonella surveillance or monitoring vaccine efficacy. This work was carried out to identify novel immunogenic proteins which would be useful in developing ELISA based diagnostic assays with a higher specificity for the diagnosis of Salmonella serotypes responsible for human foodborne outbreaks. Twelve Salmonella isolates (3 of S.Typhimurium,3 of S. Enteritidis, 3 of S. Kentucky and 3 of S. Anatum) collected from duckling, chicken and poultry feed were obtained from the reference laboratory for veterinary quality and control on poultry production. They are tested for purity using xylose lysine deoxycholate and Confirmed by biochemical characterization, serological identification and real-time PCR. The outer membrane proteins of the tested Salmonella isolates were isolated and the protein content was analyzed by the NanoDrop®ND-1000, then those are separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.