Investigation into the Use of SeM Protein as Immunogenic Material against Equine Strangles Disease
Shaimaa Mahmoud Elsayed Abdelmageed;
Abstract
Strangles disease is caused by Streptococcus equi subspecies equi that is characterized by comprising a major virulence factor called M like protein or SeM protein.
The aim of the present study was to investigate the use of SeM protein as immunogenic material against equine strangles disease. So a locally isolated S. equi subspecies equi was obtained from Equine Bacterial Disease Lab (Animal Health Research Institute, Egypt). It was cultured on blood agar and incubated for 24 hours at 37ºC with 10%Co2. The isolate, which showed β-hemolysis, was subjected to Lancefield grouping using the Mastastrep Kit as identified as group C and biochemical identification using API20 Strep.
SeM protein extracts were prepared where SeM protein concentration was detected both in the sediment and supernatant of the prepared extract. Certain modification by further centrifugation of both the supernatant and sediment of the SeM acid extract was made. The resulting supernatants of both centrifugation were also analyzed; where four samples being analyzed electrophoretically using SDS-PAGE, including SeM acid extract sediment (SeM1), SeM acid extract supernatant (SeM2), supernatant from centrifuged SeM1 (SeM3), and supernatant from centrifuged SeM 2 (SeM4).
The sediment concentration of SeM protein was about 2.358mg/ml. While the supernatant concentration of the SeM protein was 0.364mg/ml. Electrophoresis of the extracted sediment from SeM1 revealed the appearance of a band at MW 70.9 kDa, While the electrophoresis of SeM2 revealed the presence of 7 bands at MW of 105, 87.8, 70.9, 61.1, 44, 37.9 and 18.4 kDa. Five bands were detected in SeM3 at MW 70.9, 58.9, 37.2, 29.8 and 18.3 kDa, and SeM4 showed four bands at MW of 72.0, 58.6, 29.8 and 18.0 kDa.
The aim of the present study was to investigate the use of SeM protein as immunogenic material against equine strangles disease. So a locally isolated S. equi subspecies equi was obtained from Equine Bacterial Disease Lab (Animal Health Research Institute, Egypt). It was cultured on blood agar and incubated for 24 hours at 37ºC with 10%Co2. The isolate, which showed β-hemolysis, was subjected to Lancefield grouping using the Mastastrep Kit as identified as group C and biochemical identification using API20 Strep.
SeM protein extracts were prepared where SeM protein concentration was detected both in the sediment and supernatant of the prepared extract. Certain modification by further centrifugation of both the supernatant and sediment of the SeM acid extract was made. The resulting supernatants of both centrifugation were also analyzed; where four samples being analyzed electrophoretically using SDS-PAGE, including SeM acid extract sediment (SeM1), SeM acid extract supernatant (SeM2), supernatant from centrifuged SeM1 (SeM3), and supernatant from centrifuged SeM 2 (SeM4).
The sediment concentration of SeM protein was about 2.358mg/ml. While the supernatant concentration of the SeM protein was 0.364mg/ml. Electrophoresis of the extracted sediment from SeM1 revealed the appearance of a band at MW 70.9 kDa, While the electrophoresis of SeM2 revealed the presence of 7 bands at MW of 105, 87.8, 70.9, 61.1, 44, 37.9 and 18.4 kDa. Five bands were detected in SeM3 at MW 70.9, 58.9, 37.2, 29.8 and 18.3 kDa, and SeM4 showed four bands at MW of 72.0, 58.6, 29.8 and 18.0 kDa.
Other data
| Title | Investigation into the Use of SeM Protein as Immunogenic Material against Equine Strangles Disease | Other Titles | التحقق من استخدام بروتين SeM كمادة مناعية ضد مرض خناق الخيل | Authors | Shaimaa Mahmoud Elsayed Abdelmageed | Issue Date | 2020 |
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