Nanotechnology Studies as a New Approach for Treatment of Gastric Ulcers in Male Albino Rats

Abstract

Gastric ulcers: disturbance in the gastric mucosa affect many people all over the world. It developed as an imbalance between the protective and aggressive factors. The objective of this study was designed to investigate the effect of gold nanoparticles (AuNPs) on treatment of peptic ulcers with using zinc as a conveying element to deliver the 250 µg/kg body weight (b.w.) AuNPs to the affected sites. Ulceration was induced by a single oral administration of indomethacin (IND) (30 mg/kg b.w). These treatments were carried out with zinc motor loaded with different sizes of AuNPs with green solid state laser doses. Rats were divided into nine groups (six per each). Group 1 (control group): Rats were administered orally distilled water (dist. H2O). Group 2(ulcerated rats): rats were administered a single oral dose (30 mg/kg) of IND dissolved in 5% sodium bicarbonate. Groups (3): ulcerated rats were administered single oral dose (250µg/kg.b.wt.) of zinc. Group (4 & 5): ulcerated rats were administered single oral dose of 250µg (13 nm and 42 nm) gold nanoparticles (AuNPs) respectively dissolved in aqueous solutions of zinc respectively. Group (6 & 7): ulcerated rats were administered single oral dose of 250µg (13nm and 42nm) of AuNPs dissolved in aqueous solutions with zinc and after 2 hours exposed to 532 nm green solid state laser for 10 minutes respectively. Group (8 &9): ulcerated rats were administered single oral dose of 250µg (13nm and 42nm) of AuNPs dissolved in aqueous solutions with zinc then 2 hours later exposed to 532 nm green solid state laser for 15 minutes respectively. After 7days, animals were scarified. Stomach was removed and immediately stored at –80°C for molecular, histological and biochemical analysis.