Studies On Helicobacter pylori Involved In Gastroduodenal Disorders

Abstract

Helicobacter pylori is one of the most common causes of chronic infections in human. A close relationship is now thought to exist between H pylori and peptic ulcer disease, gastritis, and certain other gastroduodenal diseases. Also, it is now firmly established that infection with H pylori is a key etiological factor in peptic ulcer disease, gastric mucosa-associated lymphoid tissue lymphoma, and gastric adenocarcinoma. Furthermore, several studies have shown that eradication of H pylori plays an important role in the treatment of some gastric-intestinal diseases. The present study was carried out on 150 patients during the period from March to August, 2004. They were "out patients" referrals to the Upper Gastrointestinal Endoscopy Unit, Suez Canal University Hospital. Each patient was subjected to endoscopic examination. Three biopsy specimens were taken from the stomach of each patient. One biopsy specimen for rapid urease test, the second for isolation and identification, and the third one for direct Gram stain. One of biopsy specimens was inoculated into Christensen's urea broth. It was examined at room temperature and after I and 24 hour incubation at 37°C. The second biopsy specimen was microscopically examined using Gram stain. The third biopsy specimen was cultured on both Colombia blood agar and Brain heart infusion agar as selective and non-selective media, respectively.