Relation of Lactobacilli Acidophilus To Obesity in Egyptian Population

Abstract

Obesity is the worldwide 21st century pandemic syndrome characterized by fat mass accumulation, mainly visceral fat. Current thinking considers a potential role of gut microbiota on the development of obesity and its related comorbidities. Moreover, probiotics are health promising microbes mainly categorized under the genus Lactobacillus e.g. Lactobacillus acidophilus and Bifidobacterium, which when administered in adequate amounts confer health benefits to the host, and have been implicated in various physiological functions including energy homeostasis and an emerging area of research. Thus, the aim of this study was to assess possible relation of lactobacilli Acidophilus to obesity in a sample of Egyptian population by real-time PCR of lactobacilli acidophilus in stool. The present study was carried out on 60 subjects who attended to outpatient clinics Kafr El-sheikh General Hospital and Ain Shams University during the period from May 2017 to June 2018. The selected subjects were divided according to body mass index (BMI) into two groups 20 health y non - obese subjects and 40 subjects who had BMI >25 kg/m2. According to the RT-PCR results, Lactobacilli acidophilus was expressed in 21 out of 40 (52.5%) fecal samples of obese cases and 16 out of 20 (80%) of fecal samples of non-obese cases (table 13). In rest of samples in both studied groups, the expression was below the detection limit. The results showed that the mean cycle threshold (CT) which Lactobacilli expressed in the obese cases was at (38.89±2.57) compared to (36.08±4.63) in non-obese cases, this mean that the expression of lactobacilli was