Fungal Productionof Lipase from AgroIndustrial Wastes Using Solid State Technique

Abstract

 Pure colonies of seventeen fungal isolates were obtained from National Research Centre and were cultivated on media containing peptone, glucose, beef extract and sodium chloride to test their capabilities to produce lipase.  Aspergillus niger NRRL599 gave the highest lipolytic activity 550U\ml followed by A.rubber gave 525± 0.7633 U/ml  Five different wastes were tested for the production of extracellular lipase at different time at static conditions.  Fermentation of olive oil waste by A.niger NRRL599 showed higher activity after 14 days in comparing to that of other wastes.  Optimum inoculum age of A.niger NRRL 599 was obtained when the organism fermented on olive oil waste and gave higher lipolytic activity (200±2.0U\ml) than wheat bran (145±1.0U\ml).  The optimum inoculum size was 4 ml (4.35 X 106/ml) of spore suspension per 100 ml medium.  The optimum concentration of waste was 5g gave 200 ±2U\ml activity.