The IMPLICATION OF (FISH) TECHNIQUE I' IN DETECTION OF CHROMOSOMAL ANOMALIES

Abstract

Florescence in situ hybridization technique (FISH) r' epresents a ' powerful and sensitive tool which can be extremely useful in I ' detecting structural and numerical chromosomal anomalies with the : following advantages: it is easy to handle, has a high success rate, is

able to detect small clones, need only a small amount of:material and , is less time consuming. It will not replace other technigues but it is however, a useful alternative screening technique that is also very I

useful to monitor patients after therapy.

The use of FISH based techniques are greatly i;mproving the ability detect genetic abnormalities including those c?' nditions with low cell proliferation. Thus, the use of FISH to detect karyotypic abnormalities is a potentially more sensitive, : proliferation­ independent technique to score cells bearing such a\mormalities at diagnosis and during the course of the disease. t also detects residual leukemic cells in apparently complete remission I