Synergistic effect of N-acetylcysteine and folic acid against aspartame- induced nephrotoxicity in rats
Afaf Abbass Sayed Saleh;
Abstract
The objective of the present study is to explore the synergic and the
presumable protective effects of the combination of N-acetyl cysteine
(NAC) and folic acid (FA) against aspartame induced nephrotoxicity.
Eighteen adult male Wistar rats, weighing 150-170 g, were randomly divided
into three groups as follows: first group was given distilled water and served
as control group; the seconed group was given aspartame dissolved in
distilled water in a dose of 500 mg /kg b.wt.; the third group was given both
NAC and folic acid dissolved in distilled water in a dose of 600 mg /kg b.wt.
and 12 mg/kg b.wt. respectively. The experimental period was carried out for
42 days. Serum urea, creatinine, and potassium were significantly increased
whereas sodium and calcium were significantly decreased in rats that had
received aspartame. Blood hemoglobin (Hb), serum iron (Fe) and ferritin
were significantly decreased whereas serum total iron binding capacity
(TIBC) and unsaturated iron binding capacity (UIBC) were significantly
increased.The concentration of reduced glutathione (GSH), and the activity
of glutathione peroxidase (Gpx), and catalase (CAT) were significantly
decreased whereas the concentration of LPO (TBARS) in kidney tissues was
significantly increased. Combined Treatment with NAC and FA significantly
restored kidney functions, modulate hematological parameters, reduced lipid
peroxidation and enhanced reduced glutathione level.
presumable protective effects of the combination of N-acetyl cysteine
(NAC) and folic acid (FA) against aspartame induced nephrotoxicity.
Eighteen adult male Wistar rats, weighing 150-170 g, were randomly divided
into three groups as follows: first group was given distilled water and served
as control group; the seconed group was given aspartame dissolved in
distilled water in a dose of 500 mg /kg b.wt.; the third group was given both
NAC and folic acid dissolved in distilled water in a dose of 600 mg /kg b.wt.
and 12 mg/kg b.wt. respectively. The experimental period was carried out for
42 days. Serum urea, creatinine, and potassium were significantly increased
whereas sodium and calcium were significantly decreased in rats that had
received aspartame. Blood hemoglobin (Hb), serum iron (Fe) and ferritin
were significantly decreased whereas serum total iron binding capacity
(TIBC) and unsaturated iron binding capacity (UIBC) were significantly
increased.The concentration of reduced glutathione (GSH), and the activity
of glutathione peroxidase (Gpx), and catalase (CAT) were significantly
decreased whereas the concentration of LPO (TBARS) in kidney tissues was
significantly increased. Combined Treatment with NAC and FA significantly
restored kidney functions, modulate hematological parameters, reduced lipid
peroxidation and enhanced reduced glutathione level.
Other data
Title | Synergistic effect of N-acetylcysteine and folic acid against aspartame- induced nephrotoxicity in rats | Authors | Afaf Abbass Sayed Saleh | Keywords | Aspartame;Nephrotoxicity; Oxidative stress;Antioxidants; Rat | Issue Date | May-2014 | Publisher | http://www.journalijar.com | Journal | International Journal of Advanced Research | ISSN | 2320-5407 |
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