A high omega-3 fatty acid diet reduces retinal lesions in a murine model of macular degeneration
Tuo, Jingsheng; Ross, Robert J; Herzlich, Alexandra A; Shen, Defen; Ding, Xiaoyan; Zhou, Min; Coon, Steven L; Hussein, Nahed Mohamed; Salem, Norman; Chan, Chi-Chao;
Abstract
Age-related macular degeneration (AMD) is one of the leading cause of blindness among the elderly; however, current therapy options are limited. Epidemiological studies have shown that a diet that is high in omega-3 polyunsaturated (n-3) fatty acids can slow disease progression in patients with advanced AMD. In this study, we evaluated the effect of such a diet on the retinas of Ccl2(-/-)/Cx3cr1(-/-) mice, a model that develops AMD-like retinal lesions that include focal deep retinal lesions, abnormal retinal pigment epithelium, photoreceptor degeneration, and A2E accumulation. Ccl2(-/-)/Cx3cr1(-/-) mice that ingested a high n-3 fatty acid diet showed a slower progression of retinal lesions compared with the low n-3 fatty acids group. Some mice that were given high levels of n-3 fatty acids had lesion reversion. We found a shunted arachidonic acid metabolism that resulted in decreased pro-inflammatory derivatives (prostaglandin E(2) and leukotriene B(4)) and an increased anti-inflammatory derivative (prostaglandin D(2)). We also measured lower ocular TNF-alpha and IL-6 transcript levels in the mice fed a diet of high n-3 fatty acids. Our findings in these mice are in line with human studies of AMD risk reduction by long-chain n-3 fatty acids. This murine model provides a useful tool to evaluate therapies that might delay the development of AMD.
Other data
Title | A high omega-3 fatty acid diet reduces retinal lesions in a murine model of macular degeneration | Authors | Tuo, Jingsheng; Ross, Robert J; Herzlich, Alexandra A; Shen, Defen; Ding, Xiaoyan; Zhou, Min; Coon, Steven L; Hussein, Nahed Mohamed ; Salem, Norman; Chan, Chi-Chao | Keywords | INFLAMMATION;POLYUNSATURATED FATTY;ACIDS;DOCOSAHEXAENOIC ACID;PIGMENT EPITHELIUM;PROSTAGLANDIN D-2;GENE-EXPRESSION;GROWTH-FACTOR;MICE;MACROPHAGES | Issue Date | Aug-2009 | Publisher | ELSEVIER SCIENCE INC | Journal | The American journal of pathology | ISSN | 0002-9440 | DOI | 10.2353/ajpath.2009.090089 | PubMed ID | 19608872 | Scopus ID | 2-s2.0-68449094580 | Web of science ID | WOS:000268508500031 |
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