Impact of Different Dietary Components on Gene Expression of Leptin and Adiponectin in Adipose Tissue of Obese Rats
Mona A. Sadek; Samah F. Darwish; Abd El-Rhman, Amira; Huda M. Ismail Abo El-Fadl;
Abstract
Aim: This study was conducted to evaluate the impact of different dietary components on gene
expression of leptin and adiponectin to understand how nutritional molecules affect gene response
and some metabolic pathways in diet-induced obesity in rats.
Methodology: Obesity was induced in adult male Spargue-Dawley rats using high fat- high
sucrose diet for 7.5 weeks. The animals were divided into 5 groups; 2 groups served as control
groups and the other 3 groups treated with the high fiber and/or high antioxidant vitamins (A & E)diets. The study involved measurement of leptin and adiponectin gene expression in adipose
tissue using PCR technique. In addition, measurement of energy intake, Lee index, serum
adipokines (adipocytokines) and some oxidative stress markers.
Results: The results indicated that dietary induction of obesity resulted in significant (P<0.05)
increase in body weight and Lee index compared with normal rats. Obesity caused significant
(P<0.05) elevation in leptin mRNA level by 57.3% and reduction in adiponectin mRNA level by
35.1% in adipose tissue compared with normal group. However, treatment of obese rats with high
fiber and antioxidant vitamins (A & E) diet caused significant (P<0.05) reduction in adipose tissue
gene expression of leptin by 27.86% with increased adiponectin mRNA level by 41.84%. A
significant (P<0.05) reduction was found in body weight and Lee index by 22.5% and 7.95%
respectively compared with control obese rats. Also serum anti-inflammatory cytokines omentin
and vaspin as well as antioxidants were increased, with reduction in pro-inflammatory cytokines as
well as malondialdehyde and nitric oxide significantly (P<0.05) as compared with their levels in
obese rats before treatment.
Conclusion: Dietary modification through reducing caloric intake and/or increasing antioxidant
vitamins (A & E) may have some effect on the adipose tissue mRNA levels of leptin and
adiponectin as well as adipocytokine serum levels related to obesity condition.
expression of leptin and adiponectin to understand how nutritional molecules affect gene response
and some metabolic pathways in diet-induced obesity in rats.
Methodology: Obesity was induced in adult male Spargue-Dawley rats using high fat- high
sucrose diet for 7.5 weeks. The animals were divided into 5 groups; 2 groups served as control
groups and the other 3 groups treated with the high fiber and/or high antioxidant vitamins (A & E)diets. The study involved measurement of leptin and adiponectin gene expression in adipose
tissue using PCR technique. In addition, measurement of energy intake, Lee index, serum
adipokines (adipocytokines) and some oxidative stress markers.
Results: The results indicated that dietary induction of obesity resulted in significant (P<0.05)
increase in body weight and Lee index compared with normal rats. Obesity caused significant
(P<0.05) elevation in leptin mRNA level by 57.3% and reduction in adiponectin mRNA level by
35.1% in adipose tissue compared with normal group. However, treatment of obese rats with high
fiber and antioxidant vitamins (A & E) diet caused significant (P<0.05) reduction in adipose tissue
gene expression of leptin by 27.86% with increased adiponectin mRNA level by 41.84%. A
significant (P<0.05) reduction was found in body weight and Lee index by 22.5% and 7.95%
respectively compared with control obese rats. Also serum anti-inflammatory cytokines omentin
and vaspin as well as antioxidants were increased, with reduction in pro-inflammatory cytokines as
well as malondialdehyde and nitric oxide significantly (P<0.05) as compared with their levels in
obese rats before treatment.
Conclusion: Dietary modification through reducing caloric intake and/or increasing antioxidant
vitamins (A & E) may have some effect on the adipose tissue mRNA levels of leptin and
adiponectin as well as adipocytokine serum levels related to obesity condition.
Other data
Title | Impact of Different Dietary Components on Gene Expression of Leptin and Adiponectin in Adipose Tissue of Obese Rats | Authors | Mona A. Sadek; Samah F. Darwish; Abd El-Rhman, Amira ; Huda M. Ismail Abo El-Fadl | Keywords | Adipokines;obesity;nutrigenomics;anthropometric measurements;gene expression;PCR | Issue Date | Jan-2017 | Journal | International Journal of Biochemistry Research& Review | Volume | 15 | Issue | 2 | DOI | 10.9734/IJBCRR/2016/30489 |
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