THE PROTECTIVE EFFECT OF BEETROOT AND CARROT JUICES ON DOXORUBICIN-INDUCED CARDIAC INJURY AND ASSOCIATED INFLAMMATORY RESPONSE

Abstract

ABSTRACT Doxorubicin (Dox), has an importance as antitumor antibiotic, but it’s using is limited by the development of cardiomyopathy. Free radicals are involved in acute doxorubicin-induced toxicity and inflammatory condition in cardiac tissue. The study aimed to determine the protective effect of beetroot and/or carrot juices in acute Dox induced cardiac injury in adult male rats. Animals were randomly separated into 5 groups, G1control received normal saline I.P, G2 received single dose of Dox 20 mg/kg I.P, the other three groups also received doxorubicin 20 mg/kg I.P as single dose after four weeks cumulative doses of beetroot juice (10 mg/kg), carrot juice (10 mg/kg) and mixture of equal volumes of two juices respectively. Serum CK-MB, LDH, C- RP, Homocysteine and cardiac troponin-I were examined by ELIZA. Heart tissue histopathology, DNA damage of cardiac cell and oxidative stress and inflammatory markers were determined in all rats after 48hrs from doxorubicin injection. Doxorubicin induced cardiac injury was evidenced by a significant elevation in serum markers of heart function (CK-MB, LDH, Homocysteine and cardiac troponin-I), oxidative stress and inflammatory markers (MDA, CAT, IL-6 and CRP). Also cardiac cell’s DNA damage was indicated by significant changes in comet assay parameters. All these results was confirmed by heart tissue histopathology. While, administration of beetroot and carrot juices protect heart tissue from damage by oxidative and inflammatory condition. Moreover mixing of two juices was more effective.