PROTECTIVE EFFECTS OF SULFORAPHANE AND/OR SELENIUM NANOPARTICLES AS WELL AS BROCCOLI JUICE ON ALCL3 INDUCED ALZHEIMER'S DISEASE IN RATS
Ola Mohamed Samy Ahmad Sadek; Abd El-Rhman, Amira; Nehad Naem Hamed Shosha; Gehan Salah Eldin Moram Ali; Azza Abd El Fattah Ali; Nahla Hussein Ali Ali;
Abstract
Objectives: We examined the effects of Sulforaphane (SFN) and/or
Selenium (Se) nanoparticles (NPs) as well as fresh broccoli juice
(FBJ), on metabolic, epigenetic, antioxidant, inflammatory and
apoptotic markers in Alzheimer’s disease (AD) induced by AlCl3 in
rats. Methods: After characterization of the NPs and the determination
of FBJ’s bioactive components, sixty male Sprague Dawley rats were
divided into 6 groups (G1: healthy control, G2: untreated AD rats, G3:
SFN NPs, G4: Se NPs, G5: SFN +Se NPs and G6: FBJ). The
nutraceuticals were used as pre-and post-AD treatments; rats were first
given oral doses of the tested neutraceticals for 2 weeks then received
daily i.p. injections of AlCl3.6H2O (70 mg/kg/day) to induce AD, and
continued to receive the tested nutraceuticals along with the AlCl3 for
the next 5 weeks. Results: Our pre-and post-AD treatments
significantly improved rats’ performances in Morris water maze task and the conditioned
avoidance test. SFN NPs were the most effective, by virtue of their small size and
characteristic, followed by FBJ. Epigenetic and antioxidant effects followed the same
direction and were apparent through significantly halting the effects of AlCl3 on histone
deacetylase activity as well as the gene expression of DNA methyltransferase-1 and heme
oxygenase-1, further protecting the downstream endogenous antioxidant system. Levels ofmetabolic dysfunction, inflammation and apoptosis markers also reflected the neuroprotective effects of our pre-and post-AD treatments. These results were confirmed by histological and immunohistochemical examinations. Conclusion: All tested pre-and post-AD treatments significantly exhibited neuroprotective actions however, SFN NPs were the most effective.
Selenium (Se) nanoparticles (NPs) as well as fresh broccoli juice
(FBJ), on metabolic, epigenetic, antioxidant, inflammatory and
apoptotic markers in Alzheimer’s disease (AD) induced by AlCl3 in
rats. Methods: After characterization of the NPs and the determination
of FBJ’s bioactive components, sixty male Sprague Dawley rats were
divided into 6 groups (G1: healthy control, G2: untreated AD rats, G3:
SFN NPs, G4: Se NPs, G5: SFN +Se NPs and G6: FBJ). The
nutraceuticals were used as pre-and post-AD treatments; rats were first
given oral doses of the tested neutraceticals for 2 weeks then received
daily i.p. injections of AlCl3.6H2O (70 mg/kg/day) to induce AD, and
continued to receive the tested nutraceuticals along with the AlCl3 for
the next 5 weeks. Results: Our pre-and post-AD treatments
significantly improved rats’ performances in Morris water maze task and the conditioned
avoidance test. SFN NPs were the most effective, by virtue of their small size and
characteristic, followed by FBJ. Epigenetic and antioxidant effects followed the same
direction and were apparent through significantly halting the effects of AlCl3 on histone
deacetylase activity as well as the gene expression of DNA methyltransferase-1 and heme
oxygenase-1, further protecting the downstream endogenous antioxidant system. Levels ofmetabolic dysfunction, inflammation and apoptosis markers also reflected the neuroprotective effects of our pre-and post-AD treatments. These results were confirmed by histological and immunohistochemical examinations. Conclusion: All tested pre-and post-AD treatments significantly exhibited neuroprotective actions however, SFN NPs were the most effective.
Other data
Title | PROTECTIVE EFFECTS OF SULFORAPHANE AND/OR SELENIUM NANOPARTICLES AS WELL AS BROCCOLI JUICE ON ALCL3 INDUCED ALZHEIMER'S DISEASE IN RATS | Other Titles | a | Authors | Ola Mohamed Samy Ahmad Sadek; Abd El-Rhman, Amira ; Nehad Naem Hamed Shosha; Gehan Salah Eldin Moram Ali; Azza Abd El Fattah Ali; Nahla Hussein Ali Ali | Keywords | Alzheimer’s, sulforaphane, selenium, broccoli, epigenetics, antioxidants. | Issue Date | Jun-2019 | Journal | World Journal of Pharmaceutical Research | Volume | 8 | Issue | 8 | DOI | 10.20959/wjpr20198-15359 |
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