The Effect of Serratia marcescens and Genetically Improved Pseudomonas fluorescens on Meloidogyne incognita
Entsar Helmy Taha El Sayed Montaser; Abdel Razik, A. B; R.M., Shoaib; S. A. Ibrahim;
Abstract
Pseudomonas fluorescens is an effective bacteria used in
biocontrol agent for various soil borne plant diseases including
plant parasitic nematodes. The aim of this study is enhancement
the biocontrol effect of this bacterial strain by introducing
chitinase gene(s) from Serratia marcescens. A DNA fragment
containing the gene(s) were inserted into pGEM vector to
construct a new plasmid, which introduced into P. fluorescens.
Restriction enzyme digestion and chitin plate culture confirmed
that the improved P. fluorescens is containing a functional
chitinase gene(s). Bioassay comparative results among P.
fluorescens wild type, Serratia marcescens and transformed P.
fluorescens which conducted in screen house experiment indicated
that transformed P. fluorescens had increased effect against plant
parasitic nematodes (Meloidogyne incognita)
biocontrol agent for various soil borne plant diseases including
plant parasitic nematodes. The aim of this study is enhancement
the biocontrol effect of this bacterial strain by introducing
chitinase gene(s) from Serratia marcescens. A DNA fragment
containing the gene(s) were inserted into pGEM vector to
construct a new plasmid, which introduced into P. fluorescens.
Restriction enzyme digestion and chitin plate culture confirmed
that the improved P. fluorescens is containing a functional
chitinase gene(s). Bioassay comparative results among P.
fluorescens wild type, Serratia marcescens and transformed P.
fluorescens which conducted in screen house experiment indicated
that transformed P. fluorescens had increased effect against plant
parasitic nematodes (Meloidogyne incognita)
Other data
| Title | The Effect of Serratia marcescens and Genetically Improved Pseudomonas fluorescens on Meloidogyne incognita | Authors | Entsar Helmy Taha El Sayed Montaser ; Abdel Razik, A. B; R.M., Shoaib; S. A. Ibrahim | Keywords | Genetic transformation Pseudomonas fluorescens Serratia marcescens Chitinase gene Meloidogyne incognita Biological control | Issue Date | 27-May-2016 | Publisher | Egyptian Academic Journal of Biological Sciences B. Zoology ISSN: 2090 – 0759 www.eajbs.eg.net | Journal | Egyptian Academic Journal of Biological Sciences B. Zoology ISSN: 2090 – 0759 | Volume | 8 | Issue | 1 | Start page | 49 | End page | 59 |
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