New Approaches For Extracting And Mass Rearing of Entomopathogenic Nematodes In Vivo
Entsar Helmy Taha El Sayed Montaser; A.S. Kassab;
Abstract
The dual-purpose of spongy traps for extracting and storage of
EPNs was validated in this investigation. The spongy traps proved to be
the most efficient method for recovering IJs from the insect cadavers
(Galleria mellonella) comparing with the traditional white traps. Also,
the spongy trap kept the viability of IJs for prolonged time (12 weeks).
Based on these results, duplications of insect cadavers in spongy traps
will be rather encouraging for nematode mass production. A linked study
was continued for detection an artificial diet for G. mellonella larvae.
This step is necessary towards the mass production of EPNs in vivo. The
recommended artificial media divided into two parts: the first part is
supplemented with old beewax used for feeding 1st and 2nd instars of
insect larvae. The second part is supplemented with paraffin oil used for
feeding the other instars of insect larvae. The present results
demonstrated that, the insect larvae could live and develop on this
artificial diet. Moreover, both nematodes of Heterorhabditis
bacteriophora (Hb) and Steinernema carocapcae (Sc) have proven to
propagate perfectly in insects fed on the artificial media. Full death of all
treated insects by Sc or Hb occurred (mortality=100%). The
pathogenicity and reproduction of Hb nematodes produced from insect
larvae fed on artificial diet gave initial population (Pi), final population
(Pf), Rate of reproduction (Rr) and Efficiency of conversion (Ec) values
superior than those fed on beewax checks at 100 IJs/insect larva.
Similary, the pathogenicity and reproduction of Sc nematodes produced
from insect larval fed on artificial diet were equivalent to or superior than
those produced from insects fed on beewax in all inoculum levels. These
new methods require minimal expertise and capital investments for
extracting, storage and mass production of the beneficial
Entomopathogenic nematodes (EPNs).
EPNs was validated in this investigation. The spongy traps proved to be
the most efficient method for recovering IJs from the insect cadavers
(Galleria mellonella) comparing with the traditional white traps. Also,
the spongy trap kept the viability of IJs for prolonged time (12 weeks).
Based on these results, duplications of insect cadavers in spongy traps
will be rather encouraging for nematode mass production. A linked study
was continued for detection an artificial diet for G. mellonella larvae.
This step is necessary towards the mass production of EPNs in vivo. The
recommended artificial media divided into two parts: the first part is
supplemented with old beewax used for feeding 1st and 2nd instars of
insect larvae. The second part is supplemented with paraffin oil used for
feeding the other instars of insect larvae. The present results
demonstrated that, the insect larvae could live and develop on this
artificial diet. Moreover, both nematodes of Heterorhabditis
bacteriophora (Hb) and Steinernema carocapcae (Sc) have proven to
propagate perfectly in insects fed on the artificial media. Full death of all
treated insects by Sc or Hb occurred (mortality=100%). The
pathogenicity and reproduction of Hb nematodes produced from insect
larvae fed on artificial diet gave initial population (Pi), final population
(Pf), Rate of reproduction (Rr) and Efficiency of conversion (Ec) values
superior than those fed on beewax checks at 100 IJs/insect larva.
Similary, the pathogenicity and reproduction of Sc nematodes produced
from insect larval fed on artificial diet were equivalent to or superior than
those produced from insects fed on beewax in all inoculum levels. These
new methods require minimal expertise and capital investments for
extracting, storage and mass production of the beneficial
Entomopathogenic nematodes (EPNs).
Other data
Title | New Approaches For Extracting And Mass Rearing of Entomopathogenic Nematodes In Vivo | Authors | Entsar Helmy Taha El Sayed Montaser ; A.S. Kassab | Keywords | Extracting storage EPNs Heterorhabditis bacteriophora Steinernema carocapcae Galleria mellonella artificial media | Issue Date | 27-Apr-2016 | Publisher | Egyptian Academic Journal of Biological Sciences B. Zoology | Journal | Egyptian Academic Journal of Biological Sciences B. Zoology | Volume | 8 | Issue | 1 | Start page | 39 | End page | 47 |
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