Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel

Al-Huqail, Asma A.; Nagwa Mohamed Amin Aref;

Abstract


Programed cell death resembles a real nature active defense in Datura metel against TMV after three days of virus infection. This adaptive plant immune response was quantitatively assessed against Tomato Mosaic Virus infection by the following physiological markers; Chlorophyll-a (mg/g), Chlorophyll-b (mg/g), total protein (mg/g), hydrogen peroxide H O (μmol/100 mg), DNA (μg/100 mg), RNA (μg/100 mg), Salicylic acid (μg/g), and Comet Assays. Parameters were assessed for asymptomatic healthy and symptomatic infected detached leaves. The results indicated H O and Chlorophyll-a as the most potential parameters. Chlorophyll-a was considered the only significant predictor variant for the H O dependent variant with a P value of 0.001 and R-square of 0.900. The plant immune response was measured within three days of virus infection using the cutoff value of H O (⩽1.095 μmol/100 mg) and (⩽3.201 units) for the tail moment in the Comet Assay. Their percentage changes were 255.12% and 522.40% respectively which reflects the stress of virus infection in the plant. Moreover, H O showed 100% specificity and sensitivity in the symptomatic infected group using the receiver-operating characteristic (ROC). All tested parameters in the symptomatic infected group had significant correlations with twenty-five positive and thirty-one negative correlations where the P value was <0.05 and 0.01. Chlorophyll-a parameter had a crucial role of highly significant correlation between total protein and salicylic acid. Contrarily, this correlation with tail moment unit was (r = −0.930, P < 0.01) where the P value was <0.01. The strongest significant negative correlation was between Chlorophyll-a and H O at P < 0.01, while moderate negative significant correlation was seen for Chlorophyll-b where the P value < 0.05. The present study discloses the secret of the three days of rapid transient production of activated oxygen species (AOS) that was enough for having potential quantitative physiological parameters for defensive plant response toward the virus. 2 2 2 2 2 2 2 2 2 2 2 2


Other data

Title Physiological parameters correlated with Tomato Mosaic Virus inducing defensive response in Datura metel
Authors Al-Huqail, Asma A.; Nagwa Mohamed Amin Aref 
Keywords Chlorophyll-a | Datura metel | Hydrogen peroxide (H O ) 2 2 | Physiological parameters | Plant-adaptive immune response | Programed cell death | Tail moment unit | TMV
Issue Date 1-May-2017
Publisher ELSEVIER
Journal Saudi Journal of Biological Sciences 
ISSN 1319562X
DOI 10.1016/j.sjbs.2016.04.003
PubMed ID 28490959
Scopus ID 2-s2.0-84964959903
Web of science ID WOS:000402476100017

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