Curative role of lactulose, L-carnitine, alpha-lipoic acid and combination of L-carnitine and alpha-lipoic acid in a rat model of acute hepatic encephalopathy : Biochemical observations
Afaf Abbass Sayed Saleh; Saeed, Rokaya; Hanaa Hamdy Ahmed; Yassmen Said Ahmed;
Abstract
Purpose: To determine whether a combination L-carnitine and α-lipoic acid (ALA) can alleviate the toxic
effects of thioacetamide (TAA) via their potent antioxidant and free radical-scavenging activities.
Methods: Rats were injected with TAA for 3 days to induce acute hepatic failure. TAA induced rats
were treated with each of lactulose, L-carnitine and ALA alone and a combination of L-carnitine and
ALA for 3 months. Thereafter, biochemical indices, ammonia levels, oxidative stress markers, and the
levels of inflammatory markers were assessed in serum , liver and brain.
Results: A significant improvement was observed after 3 months of antioxidants treatment. Lactulose,
L-carnitine and ALA significantly decreased serum concentrations of alanine transaminase (ALT),
aspartate aminotransaminase (AST) and level of total bilirubin while both levels of total protein (TP) and
albumin (ALB) were significantly increased (p < 0.05 ) compared to TAA group. In addition, each of
antioxidants alone significantly decreased ammonia (NH3) concentrations of serum, liver and brain in
TAA-induced rats. Treatment with antioxidants for 3 months significantly (p < 0.05) decreased
Malondialdehyde (MDA) and nitric oxide (NO) while antioxidant enzyme activities of glutathione
peroxidase (GPX) and superoxide dismutase (SOD) were significantly increased (p < 0.05) in liver and
brain tissues. The expressions of serum tumor necrosis factor-α (TNF-α) and soluble protein (S100-β)
were significantly (p < 0.05) down-regulated in TAA-induced rats.
Conclusion: L-carnitine in combination with ALA can mitigate HE induced experimentally in rats. The
protective efficacy of L-carnitine in combination with ALA in HE can be attributed to suppression of
oxidative stress, ammonia concentration and the levels of inflammatory markers. Thus, it may have the
potential to be used to treat liver cirrhosis in clinical settings.
effects of thioacetamide (TAA) via their potent antioxidant and free radical-scavenging activities.
Methods: Rats were injected with TAA for 3 days to induce acute hepatic failure. TAA induced rats
were treated with each of lactulose, L-carnitine and ALA alone and a combination of L-carnitine and
ALA for 3 months. Thereafter, biochemical indices, ammonia levels, oxidative stress markers, and the
levels of inflammatory markers were assessed in serum , liver and brain.
Results: A significant improvement was observed after 3 months of antioxidants treatment. Lactulose,
L-carnitine and ALA significantly decreased serum concentrations of alanine transaminase (ALT),
aspartate aminotransaminase (AST) and level of total bilirubin while both levels of total protein (TP) and
albumin (ALB) were significantly increased (p < 0.05 ) compared to TAA group. In addition, each of
antioxidants alone significantly decreased ammonia (NH3) concentrations of serum, liver and brain in
TAA-induced rats. Treatment with antioxidants for 3 months significantly (p < 0.05) decreased
Malondialdehyde (MDA) and nitric oxide (NO) while antioxidant enzyme activities of glutathione
peroxidase (GPX) and superoxide dismutase (SOD) were significantly increased (p < 0.05) in liver and
brain tissues. The expressions of serum tumor necrosis factor-α (TNF-α) and soluble protein (S100-β)
were significantly (p < 0.05) down-regulated in TAA-induced rats.
Conclusion: L-carnitine in combination with ALA can mitigate HE induced experimentally in rats. The
protective efficacy of L-carnitine in combination with ALA in HE can be attributed to suppression of
oxidative stress, ammonia concentration and the levels of inflammatory markers. Thus, it may have the
potential to be used to treat liver cirrhosis in clinical settings.
Other data
| Title | Curative role of lactulose, L-carnitine, alpha-lipoic acid and combination of L-carnitine and alpha-lipoic acid in a rat model of acute hepatic encephalopathy : Biochemical observations | Authors | Afaf Abbass Sayed Saleh ; Saeed, Rokaya ; Hanaa Hamdy Ahmed ; Yassmen Said Ahmed | Keywords | Lactulose, L-carnitine, α-Lipoic acid, Hepatic encephalopathy, Thioacetamide, Oxidative stress, Cirrhosis, Acute liver injury | Issue Date | 16-Sep-2017 | Publisher | Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. | Journal | Tropical Journal of Pharmaceutical Research | DOI | 10.4314/tjpr.v16i9.17 |
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