Serum Th17 cytokines in leprosy: correlation with circulating CD4(+) CD25 (high)FoxP3 (+) T-regs cells, as well as down regulatory cytokines
Attia, E A S; El-Khateeb, Ekramy; Saad, A A; Lotfi, R A; El-Shennawy, D; Abdallah, M.M.F.;
Abstract
Leprosy is not only a bacteriological disease but also an immunological disease, in which T helper17 and CD4(+) CD25(high)FoxP3(+) regulatory T cells (T-regs), among others, may play a role. We aimed to evaluate serum levels of interleukin (IL)-17, IL-22 (Th17 cytokines), IL-10 and transforming growth factor (TGF)-β (down regulatory cytokines) in 43 untreated leprosy patients and 40 controls by enzyme-linked immunosorbent assay, and to assess circulating CD4(+) CD25(high)FoxP3(+)T-regs in patients using flow cytometry. Patients were grouped into tuberculoid, pure neural, borderline, lepromatous, type 1 reactional leprosy, and erythema nodosum leprosum. IL-10 and TGF-β were significantly higher in patients as compared to controls (p < 0.001), while IL-17, but not IL-22, was significantly lower (p < 0.001), with no significant difference comparing patients' subgroups. Significantly higher CD4(+) CD25(high)FoxP3(+)T-regs levels was detected in tuberculoid, type 1 reaction and pure neural leprosy, while the lowest levels in erythema nodosum leprosum (p < 0.001). TregsFoxP3 expression% was significantly lower in pure neural leprosy than other patients' subgroups (p < 0.05). T-regs/T-effs was lowest in erythema nodosum leprosum (p < 0.05). TGF-β correlated negatively with TregsFoxP3 expression% and T-effs% (p = 0.009 and 0.018 respectively). Leprosy is associated with defective IL-17 and overproduction of IL-10 and TGF-β. Tuberculoid, type 1 reaction and pure neural leprosy express significantly higher circulating T-regs, consistent with effector immune mechanisms activation, but with lower TregsFoxP3 expression (in pure neural leprosy). Erythema nodosum leprosum is characterized by deficient T-regs and increased TregsFoxP3 expression%. The present study pinpointed a potential role of Th17, CD4(+) CD25(high)FoxP3(+)T-regs, and probably CD4(+) CD25(+)IL-10(+) T regulatory cells 1 (Tr1), and Th3 in leprosy.
Other data
Title | Serum Th17 cytokines in leprosy: correlation with circulating CD4(+) CD25 (high)FoxP3 (+) T-regs cells, as well as down regulatory cytokines | Authors | Attia, E A S; El-Khateeb, Ekramy ; Saad, A A; Lotfi, R A; El-Shennawy, D; Abdallah, M.M.F. | Keywords | Interleukin-10;Transforming growth factor-beta;T-regs;TRANSFORMING-GROWTH-FACTOR;DIFFERENT CLINICAL FORMS;LEPROMATOUS LEPROSY;IN-VITRO;MYCOBACTERIUM-TUBERCULOSIS;IFN-GAMMA;EXPRESSION;FOXP3;RESPONSES;IL-10;Leprosy;Interleukin-22;Interleukin-17 | Issue Date | Nov-2014 | Publisher | SPRINGER | Journal | Archives of dermatological research | Volume | 306 | Issue | 20 | Start page | 793 | End page | 801 | ISSN | 0340-3696 | DOI | 10.1007/s00403-014-1486-2 | PubMed ID | 25018055 | Scopus ID | 2-s2.0-84920990510 | Web of science ID | WOS:000344168300003 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.