Comparative study of Quercetin or/and Urate Oxidase against Gentamicin -induced Nephrotoxicity and Oxidative Stress in Rat kidneys

Abstract

This study was conducted to show whether quercetin and urate oxidase would offer ameliorating effects against abnormal alterations in kidney function tests in gentamicin induced nephrotoxic rats. Two experiments were carried out, the first one showed that daily injection of 80 mg gentamicin /kg b. wt interaperitonealy (I.P) for two weeks induced acute renal failure indicated by significant elevation in serum levels of urea, creatinine, uric acid, potassium, inorganic phosphorus and Parathyroid hormone (PTH) and a significant decline in serum sodium, total and ionized calcium associated with a remarkable decrease in the content of glutathione (GSH) and in the activities glutathione peroxidase (Gpx) and catalase (CAT) and in the concentration of thiobarbituric acid reactive substances (TBARS) in the kidney of nephrotoxic rats when compared with their corresponding values in saline injected rats (Normal animals group). In the second experiment, four comparisons were made between gentamicin induced nephrotoxic rats and other nephrotoxic groups received daily i.p. injection of quercetin (50mg/kg b.wt) and urate oxidase (10mg/kg b. wt) for 2 & 4 weeks after the incidence of nephrotoxicity. A remarkable correction was occurred in the levels of serum urea, creatinine, uric acid, potassium, sodium, total and ionized calcium, inorganic phosphorus and PTH in quercetin or urate oxidase treated groups exhibited significant reduction than nephrotoxic untreated rats dependent on the time of treatment (2 & 4 weeks). In the kidney tissues, a considerable amelioration effect was occurred in the content of the levels of GSH and in the activities Gpx and CAT and in the concentration of TBARS after the nephrotoxic rats treated with quercetin or urate oxidase. These corrections were dependent on the time of treatment (2 & 4 weeks). Thus, it may be concluded that quercetin or urate oxidase can be applicable as therapeutic agent with gentamicin therapy. The best beneficial effect was more prominent when nephrotoxic rats treated with both agents (quercetin or urate oxidase) at last interval (4 weeks). The obtained data were discussed according to available obtained researches.