Kinetic behavior of Candida tropicalis during xylitol production using semi-synthetic and hydrolysate based media
El Baz, AF; shetaia, yousseria; Elkhouli, RR;
Abstract
The effects of the xylose concentration and the air supply on the production of xylitol from D-xylose by the yeast Candida tropicalis (AY 2007) using semi-synthetic and hydrolysate based media were investigated in four fermentation batch runs. The xylitol formation was favored under oxygen-limited conditions (2nd experiment, air flow rate 0.3 vvm) with a maximum xylitol production (36.0gm/l) after 59h as compared with that obtained in the 1st experiment (30.99 g/L) after 66 h under relatively less oxygen limited condition (air flow rate 1.5 vvm). Increasing the initial xylose concentration from 45.5 g/L in the 2nd experiment to 96.9 g/L in the 3rd experiment led to a great decrease in the xylitol accumulation rate (Qp) from 0.506 to 0.134 g/L, xylitol yield coefficient (Yp/s) from 0.704 to 0.180 g/g and a mild increase in the xylose consumption rate (Qs) from 0.70 to 0.75 g/L/h. The semi-synthetic medium (1st and 2nd experiments) had a maximum xylitol yield coefficient (Yp/s) of 0.701 and 0.704 g/g, respectively which was less than that obtained in the hydrolysate experiment (0.783 g/g). This is probably due to the low hydrolysate initial xylose content (29.8 g/L). © 2011 Academic Journals.
Other data
Title | Kinetic behavior of Candida tropicalis during xylitol production using semi-synthetic and hydrolysate based media | Authors | El Baz, AF; shetaia, yousseria ; Elkhouli, RR | Keywords | Xylitol;Candida tropicalis;xylose;pH;aeration;kinetics;D-XYLOSE;BAGASSE HYDROLYSATE;FERMENTATION;YEASTS | Issue Date | 2011 | Publisher | ACADEMIC JOURNALS | Journal | AFRICAN JOURNAL OF BIOTECHNOLOGY | ISSN | 1684-5315 | DOI | 10.5897/AJB11.1766 | Scopus ID | 2-s2.0-81855218980 | Web of science ID | WOS:000298558500031 |
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