Phenolic constituents, antimicrobial, antioxidant, and anticancer activities of ethyl acetate and n-butanol extracts of senna italica
Khalaf, Omar M.; Ghareeb, Mosad A.; Saad, Amal M.; Hassan M. F. Madkour; El-Ziaty, Ahmed K.; Abdel-Aziz, Mohamed S.;
Abstract
Different solvent extracts of the aerial parts of Senna italica (Mill.) were investigated for their chemical constituents and biological activities. Moreover, bio-guided fractionation led to isolation and identification of six compounds, namely: physcion (1), emodin (2), 2-methoxy-emodin-6-O-D-glucopyranoside (3), 1-hydroxy-2-acetyl-3-methyl-6-hydroxy-8-methoxynaphthalene (tinnevellin) (4), quercetin 3-O-L-rhamnopyranosyl-(16)-D-glucopyranoside (rutin) (5), and 1,6,8-trihydroxy-3-methoxy-9,10-dioxo-9,10-dihydroanthracene (6). The chemical structures of these compounds were established via 1D and 2D 1H-and 13C-NMR spectroscopy. Ethyl acetate and n-butanol extracts as well as compound 3 were evaluated for their anticancer activity against tumor cell lines. The tested extracts showed a moderate to weak activity, while compound 3 showed a moderate activity against human liver cancer (Hep G2) and breast cancer (MCF-7) cell lines with IC50 values of 57.5 and 42.3 g/mL, respectively. Both ethyl acetate and n-butanol extracts exhibited antimicrobial activities with different strengths, i.e., ethyl acetate exhibited antimicrobial activity against seven test microbes while n-butanol extract showed antimicrobial activity against all tested microbes. This is the first report for the isolation of compound 3 as a new compound from S. italica growing in Egypt.
Other data
Title | Phenolic constituents, antimicrobial, antioxidant, and anticancer activities of ethyl acetate and n-butanol extracts of senna italica | Authors | Khalaf, Omar M.; Ghareeb, Mosad A.; Saad, Amal M.; Hassan M. F. Madkour ; El-Ziaty, Ahmed K.; Abdel-Aziz, Mohamed S. | Keywords | ABTS;Anthraquinones;Anticancer;Antimicrobial;Senna italica | Issue Date | 1-Jun-2019 | Journal | Acta Chromatographica | Volume | 31 | Issue | 2 | Start page | 138 | End page | 145 | ISSN | 12332356 | DOI | 10.1556/1326.2018.00412 | Scopus ID | 2-s2.0-85067363217 |
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