Investigation of the regenerative potential of necrotic mature teeth following different revascularisation protocols
Fahmy, Sarah Hossam; Hassanien, Ehab El Sayed; Nagy, Mohamed; El Batouty, Kariem Mostafa; Mekhemar, Mohamed; Fawzy El Sayed, Karim; Hassanein, El Hassan; Wiltfang, Jörg; Dörfer, Christof;
Abstract
This study aimed to assess the revascularisation potential of necrotic mature teeth in a dog model following different protocols. Periapical infection was induced in 54 mature premolars. Teeth were distributed into seven groups: (1) Double-antibiotic-paste/Blood clot, (2) Ciprofloxacin/collagen, (3) Double-antibiotic-paste/Collagen, (4) Modified Tri-antibiotic-paste /collagen, (5) Ciprofloxacin/Gelfoam, (6) Double-antibiotic-paste/Gelfoam, and (7) Modified Triantibiotic- paste/Gelfoam. Positive and negative controls included infected and healthy teeth, respectively, (n = 12 roots/group). Canals were apically shaped to size 0.6 mm then disinfected for 1 month. Intra-canal bleeding was induced then scaffolds were applied for another month. Teeth and supporting bone were surgically sampled. Tissues were histologically scored and vimentin immuno-intensity was estimated. Ciprofloxacin and Double-antibiotic paste/Collagen resulted in significantly better corono-apical tissue ingrowths, vascularity, cementum formation and significantly lower inflammatory extents (P < 0.05).These groups also showed significantly higher Vimentin intensities, (P < 0.05). The applied protocols revascularised necrotic mature canals and reduced inflammation particularly in the Ciprofloxacin/collagen and Double-antibiotic-paste/collagen groups.
Other data
Title | Investigation of the regenerative potential of necrotic mature teeth following different revascularisation protocols | Authors | Fahmy, Sarah Hossam; Hassanien, Ehab El Sayed; Nagy, Mohamed ; El Batouty, Kariem Mostafa; Mekhemar, Mohamed; Fawzy El Sayed, Karim; Hassanein, El Hassan; Wiltfang, Jörg; Dörfer, Christof | Keywords | intra-canal disinfection;mature teeth;regenerative endodontic therapy;revascularisation;scaffolds | Issue Date | Aug-2017 | Publisher | WILEY | Journal | Australian endodontic journal : the journal of the Australian Society of Endodontology Inc | Volume | 43 | Issue | 2 | Start page | 73 | End page | 82 | ISSN | 1329-1947 | DOI | 10.1111/aej.12210 | PubMed ID | 28766808 | Scopus ID | 2-s2.0-85026639522 | Web of science ID | WOS:000409198000003 |
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