Indirect Regeneration Tissue Culture and Molecular Characterization for Some Sugar Beet (Beta vulgaris L.) Genotypes

Abstract

Sugar beet seeds are imported from European countries with expensive cost as well as many tries were procedured for flowering and seeds production but without any outcome, therefore that the use of tissue culture technique is a useful tool in Egypt. The investigation was carried out during 2017- 2018 at the laboratories of the cell research department, field crops research institute, agricultural research center, An indirect regeneration protocol for micropropagation of some sugar beet (Beta vulgaris L.) genotypes and the genetic variation were described. For callus production, hypocotyl, cotyledon explants were cultured on Murashige and Skoog’s medium supplemented with 0.1 mg/l BAP+2.0 mg/l 2,4-D and cotyledon explants produced significantly more calli than hypocotyl explants. Significant difference among the genotypes and among the different media was observed. For shoot induction callus of different explants was transferred to MS-medium containing 1.0 mg/l BAP+0.2 mg/l IAA. The regenerated shoots were transferred to rooting MS-medium containing 3.0 mg/l IBA+0.3 mg/l NAA. The genetic variation among the sugar beet genotypes was based on RAPD- PCR data suggest a relatively high genetic diversity among them, that can be used in the breeding programs taking into account select the parents, which have the wide genetic distance.