Isolation of anti-fungal agent from a soil inhabitant Streptomyces albaduncus-M51 and its efficacy against osmophilic food spoilage by Saccharomyces cerevisiae
Magda Magdy Abd El-Salam; Yassin, Sahar;
Abstract
Aim: This study was performed to isolate antifungal substance from actinomycetes
species found in soil in order to avoid osmophilic food spoilage. Methods: Purified
isolates of different actinomycetes collected from soil in Makkah region, Saudi Arabia
were screened for their antifungal bioactivity against the unicellular fungal strain,
Saccharomyces cerevisiae. The actinomycete isolate that showed the highest fungal
inhibition potency was selected and identified. Antifungal metabolite fermented by
identified isolate was extracted and minimum inhibitory concentration (MIC) was
determined. Food preservative efficacy of the extracted crude fungicide was tested
against osmophilic foods spoilage. Results: Among 59 actinomycetes strains, 10
isolates exhibited antifungal efficacy against S. cerevisiae. Only one isolate was the
most potent fungicidal and identified as Streptomyces albaduncus-M51. Crude
antifungal was extracted and MIC value found to be 25μg/ml against the tested yeast
strain. Yeast colonies were completely undetected in treated food samples at crude
extract concentration (150μl/100ml for liquid foods and 200μl/100mg for solid
samples). Conclusion: The antifungal agent produced by S.albaduncus-M51
demonstrated an obvious inhibitory effect against S. cerevisiae that causes osmophilic
foods spoilage.
species found in soil in order to avoid osmophilic food spoilage. Methods: Purified
isolates of different actinomycetes collected from soil in Makkah region, Saudi Arabia
were screened for their antifungal bioactivity against the unicellular fungal strain,
Saccharomyces cerevisiae. The actinomycete isolate that showed the highest fungal
inhibition potency was selected and identified. Antifungal metabolite fermented by
identified isolate was extracted and minimum inhibitory concentration (MIC) was
determined. Food preservative efficacy of the extracted crude fungicide was tested
against osmophilic foods spoilage. Results: Among 59 actinomycetes strains, 10
isolates exhibited antifungal efficacy against S. cerevisiae. Only one isolate was the
most potent fungicidal and identified as Streptomyces albaduncus-M51. Crude
antifungal was extracted and MIC value found to be 25μg/ml against the tested yeast
strain. Yeast colonies were completely undetected in treated food samples at crude
extract concentration (150μl/100ml for liquid foods and 200μl/100mg for solid
samples). Conclusion: The antifungal agent produced by S.albaduncus-M51
demonstrated an obvious inhibitory effect against S. cerevisiae that causes osmophilic
foods spoilage.
Other data
Title | Isolation of anti-fungal agent from a soil inhabitant Streptomyces albaduncus-M51 and its efficacy against osmophilic food spoilage by Saccharomyces cerevisiae | Authors | Magda Magdy Abd El-Salam ; Yassin, Sahar | Keywords | Soil; Actinomycetes; Antifungal; Osmophilic Foods; Spoilage; Saccharomyces cerevisiae; Streptomyces albaduncus-M51 | Issue Date | 1-Jul-2016 | Publisher | ScopeMed | Journal | Journal of Environmental and Occupational Science | DOI | 10.5455/jeos.20160530063639 |
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