Reconstruction of images in non‑scanned confocal microscope (NSCM) using speckle imaging

Abstract

Abstract Background: The common formation of images in CSLM assumes mechanically scanned object placed in the com‑ mon short focus of the objective lenses of the microscope, while in the arrangement under study, the scanning of the object is realized by placing a difuser behind the collimating lens. A model is suggested in the formation of images in Confocal Scanning Laser Microscope (CSLM) using non-scanned object. Since the illumination and detection are coherent, the obtained image is constructed from the simple product of the Resultant Point Spread Function (RPSF) modulated by the difuser spread over the object transparency. Hence, the product of the object and the image of the difuser replace the mechanical scanning of the object. Results: Reconstructed images using this novel arrangement of CNSM are presented using mammographic X-ray image. Conclusions: Convolution of the RPSF and the object is realized by the spreading of the diffuser image over the object. A coherent detector captures the whole image affected by a noisy defused function. It is noted that image processing is necessary to improve noisy images by making use of filtration techniques.