Computation of the lateral and axial point spread functions in confocal imaging systems using binary amplitude mask
Hamed, Abdallah;
Abstract
In this paper, a novel aperture based on Tolardo concept composed of a
central clear disc surrounded by a series of black and white (B/W) concentric annuli of
equal transmittance is presented. Different apodized apertures of the different numbers of B/W
annuli are suggested in order to improve further the three-dimensional resolving power of
confocal imaging systems. Both the axial and lateral point spread functions (PSF) and
the corresponding irradiances are computed in both cases of conventional and confocal
scanning microscopes for the above-mentioned amplitude filters. These results of axial
and lateral irradiances are graphically represented by constructing a computer program
using MATLAB. The obtained results are compared with that obtained in the case of circular,
annular, and Martinez-Corral apodized aperture.
central clear disc surrounded by a series of black and white (B/W) concentric annuli of
equal transmittance is presented. Different apodized apertures of the different numbers of B/W
annuli are suggested in order to improve further the three-dimensional resolving power of
confocal imaging systems. Both the axial and lateral point spread functions (PSF) and
the corresponding irradiances are computed in both cases of conventional and confocal
scanning microscopes for the above-mentioned amplitude filters. These results of axial
and lateral irradiances are graphically represented by constructing a computer program
using MATLAB. The obtained results are compared with that obtained in the case of circular,
annular, and Martinez-Corral apodized aperture.
Other data
| Title | Computation of the lateral and axial point spread functions in confocal imaging systems using binary amplitude mask | Authors | Hamed, Abdallah | Keywords | Confocal imaging;lateral and axial resolutions | Issue Date | Jun-2006 | Publisher | Indian Academy of Sciences | Journal | Pramana - Journal of Physics | Volume | 66 | Issue | 6 | Start page | 1037 | End page | 1048 |
Attached Files
| File | Description | Size | Format | Existing users please Login |
|---|---|---|---|---|
| 4 phys. pramana paper 2006.pdf | 491.33 kB | Adobe PDF | Request a copy |
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.