Comparative Study Between Erythrocytes Blood Cells and Modulated Circular Aperture Using Speckle Photography

Abstract

A manipulation of four circles arranged along the Cartesian coordinates in uniform circular aperture is compared with selected four blood cells forming the object using the technique of speckle photography. The aperture model is considered to have two-fold symmetry. A diffuser is placed in the same plane of the object or the model of four circles in order to get the modulated speckle pattern which is the Fourier spectrum of object information convoluted with the Fourier spectrum of the random diffuser. The modulated speckle pattern is smeared by the Point Spread Function computed from the diffraction corresponding to the whole circular aperture. The reconstruction process gave nearly similar speckle images little degraded by the noise originating from the diffuser. The Point Spread Function corresponding to the model of four circles is computed analytically and also using the FFT technique. A comparison is made with the Fourier spectrum of the blood cells and its corresponding autocorrelation function giving nearly similar results. We can acquire the erythrocytes blood cells in the imaging plane by operating the FFT upon the modulated speckle images. The reconstructed images of blood cells are affected by the noise originating from the diffuser. A Mat-Lab code is made in the construction of the aperture and all the images are computed in the processing.