EXTRACTION AND PURIFICATION OF POLY-DIGESTIVE ENZYMES FROM SPROUTED WHEAT AND BACILLUS CEREUS AS ALTERNATIVE THERAPY FOR CELIAC PATIENTS

Abstract

Celiac disease (CD) is a disease of the digestive system resulting from the intolerance of autoimmune system against gluten and gliadin proteins in wheat, barley, rye and some varieties of oats. The aim of this work is to extract and purify protease enzymes from sprouted wheat seeds and Bacillus cereus and to evaluate their ability for the hydrolysis of both gluten and gliadin. Data indicate that the highest purity of poly-digestive protease enzymes extracted from sprouted wheat and Bacillus cereus was obtained by 0.2M trichloroacetic acid and acetone 80%, respectively. Our findings showed synergetic effect of the mixture of proteases enzyme extracted from the two sources at 1 mg/ml to hydrolyze gluten and gliadin as compared to their individual activities as well as Digestin, a positive control.