Biosynthesis of polyunsaturated fatty acids by two newly cold‑adapted Egyptian marine yeast

Abstract

The widespread awareness of polyunsaturated fatty acids (PUFAs) benefts for human health has increased the need for their commercial production. Two oleaginous yeast were isolated from the Mediterranean Sea fsh and Red Sea fsh Epinephelus aeneus and E. areolatus, respectively. These marine candidates were identifed by MALDI-TOF/MS biotyper® as Loddero myces elongisporus and Rhodotorula mucilaginosa. The efect of incubation temperature (7, 15, and 26 °C) and glucose concentration (3% and 8%) on their lipids content were investigated using Sulfo-Phospho-Vanillin (SPV) assay. Their intercel lular lipids were visualized by fuorescence microscope using Nile-Red dye. L. elongisporus and R. mucilaginosa produced 20.04% and 26.79% of Linoleic acid, respectively, on normal Basal-Defatted Medium (BDM). Linoleic acid (21.4–22.7%) and α-Linolenic acid (7.5–10.8%) were produced by R. mucilaginosa and L. elongisporus, on normal BDM at 15 °C. High-Glucose BDM induced a positive efect on the total lipids production that reached its maximum of 48% and 54% by R. mucilaginosa and L. elongisporus, respectively, grown at 15 °C. Remarkably, 12.12% of long-chain 15-Docosenoic acid (C22:1) and 21.49% of Tricosanoic acid (C23:0) were detected in the FAs profle of L. elongisporus, when grown on normal BDM at 26 °C. The present study is the frst one reporting the FAs profle of the Egyptian Marine L. elongisporus, and its capability to accumu late high amounts of lipids under appropriate fermentation conditions; thus, it could be considered for scaling up production