Bone marrow mesenchymal stem cells suppress IL-9 in adjuvant-induced arthritis
Sahar Sobhy Abd Elhalem & Nashwa Ahmed El- Shinnawy; Z. Haggag, Nawal;
Abstract
Interleukin-9 (IL-9) has been shown to be upregulated in rheumatoid arthritis (RA). The exact role of IL-
9 has not yet been effectively studied. Mesenchymal stem cells (MSCs) have shown a promising immunomodulatory
role towards repairing cartilage and restoring joint function. One of the key problems
influencing the therapeutic efficacy of stem cell therapy is the poor cell survival following transplantation.
This is attributed to oxidative and inflammatory stresses at the injured sites. Hesperidin (Hsd), a
flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-oxidant
and anti-inflammatory activities. The objective of this study is to evaluate the therapeutic paracrine
action of bone marrow MSCs on the IL-9 level in adjuvant-induced arthritis (AIA) and the
enhancement effect of Hsd on transplanted MSCs. Articular tissue inflammation and cartilage damage
were assessed by histological scoring. Antinuclear autoantibodies, tumour necrosis factor-alpha (TNF-a),
IL-9, IL-4, interferon gamma (IFN-d), and transforming growth factor-beta1 (TGF-b1), as well as malondialdehyde
(MDA), glutathione (GSH), and superoxide dismutase (SOD) levels, were assessed in spleen
tissue homogenates after treatment with MSCs either alone or combined with Hsd for 4 weeks in an
AIA rat model. Results of this study confirmed that MSCs decreased IL-9 levels in AIA and provide
novel insights into the application of Hsd on MSC-based treatments.
9 has not yet been effectively studied. Mesenchymal stem cells (MSCs) have shown a promising immunomodulatory
role towards repairing cartilage and restoring joint function. One of the key problems
influencing the therapeutic efficacy of stem cell therapy is the poor cell survival following transplantation.
This is attributed to oxidative and inflammatory stresses at the injured sites. Hesperidin (Hsd), a
flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-oxidant
and anti-inflammatory activities. The objective of this study is to evaluate the therapeutic paracrine
action of bone marrow MSCs on the IL-9 level in adjuvant-induced arthritis (AIA) and the
enhancement effect of Hsd on transplanted MSCs. Articular tissue inflammation and cartilage damage
were assessed by histological scoring. Antinuclear autoantibodies, tumour necrosis factor-alpha (TNF-a),
IL-9, IL-4, interferon gamma (IFN-d), and transforming growth factor-beta1 (TGF-b1), as well as malondialdehyde
(MDA), glutathione (GSH), and superoxide dismutase (SOD) levels, were assessed in spleen
tissue homogenates after treatment with MSCs either alone or combined with Hsd for 4 weeks in an
AIA rat model. Results of this study confirmed that MSCs decreased IL-9 levels in AIA and provide
novel insights into the application of Hsd on MSC-based treatments.
Other data
Title | Bone marrow mesenchymal stem cells suppress IL-9 in adjuvant-induced arthritis | Authors | Sahar Sobhy Abd Elhalem & Nashwa Ahmed El- Shinnawy ; Z. Haggag, Nawal | Issue Date | 2018 | Publisher | Autoimmunity | Journal | Autoimmunity | DOI | 10.1080/08916934.2018.1428956 |
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