Simultaneous determination of tianeptine and its active metabolite tianeptine MC5 in rat plasma and brain tissue using high performance liquid chromatography / electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)
Magdy Hanna Nassif, Nancy; AM El-Kosasy; Wayne D Beck; Patrick M Callahan; Alvin V Terry; Michael G Bartlett; Morsy EL Zahar, Noha;
Abstract
A selective and sensitive method was developed to simultaneously quantify tianeptine and its main active metabolite tianeptine MC5 in rat plasma and brain tissue. A one-step liquid-liquid extraction was developed for sample preparation. The linear range for plasma was from 1.0-500.0 ng mL-1 and for brain tissue it was from 1.0-500.0 ng g-1. The method was validated in accordance with US FDA guidelines (precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE) with a consistent recovery). Stable isotope-labeled tianeptine and tianeptine MC5 (tianeptine-D4 and tianeptine MC5-D4) were used as the internal standards achieving good reproducibility while reducing the matrix effects. This method was successfully applied to a preclinical study for the simultaneous determination of tianeptine and tianeptine MC5 in rat plasma and brain tissue.
Other data
Title | Simultaneous determination of tianeptine and its active metabolite tianeptine MC5 in rat plasma and brain tissue using high performance liquid chromatography / electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) | Authors | Magdy Hanna Nassif, Nancy ; AM El-Kosasy; Wayne D Beck; Patrick M Callahan; Alvin V Terry; Michael G Bartlett; Morsy EL Zahar, Noha | Issue Date | Dec-2017 | Journal | Analytical Methods | Volume | 10 | Issue | 4 | Start page | 439 | End page | 447 | ISSN | 17599660 | DOI | 10.1039/c7ay02506h | Scopus ID | 2-s2.0-85041173098 |
Attached Files
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2018-MS-Biovalidation.pdf | 562.07 kB | Unknown | View/Open |
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