Attenuated virulence of pigment-producing mutant of Aeromonas veronii bv. sobria in HeLa cells and Nile tilapia (Oreochromis niloticus)
Abolghait, Said; Mohamed, Mohamed F.; Aly, Salah M.; Garbaj, Aboubaker M.; Moawad, Ashraf A.;
Abstract
Aeromonas species are potential water/foodborne pathogens, whereas Aeromonas veronii bv. sobria is one of the most virulent species to human and fish. Most current experimental evidence has publicized that suicide plasmid dependent IS1-element untargeted integration into A. veronii bv. sobria ATCC 9071T strain was recently used to generate brown pigment-producing and spontaneous pelleting (BP+SP+) mutant. Current study was conducted to compare virulence of wild-type ATCC 9071T strain and its BP+SP+ mutant with respect to cytotoxicity in HeLa cells and lethality in Nile tilapia. It was found that the cytotoxicity of wild-type ATCC 9071T strain to HeLa cells has reached 75% versus 50% for the cytotoxicity of BP+SP+ mutant. Further, the median lethal dose (LD50) of wild-type ATCC 9071T strain in Nile tilapia was 8.25 Log10 colony-forming units (CFU)/ml, compared to 9.16Log10CFU/ml for the LD50 of BP+SP+ mutant. Thus, current study supports the notion that non pigment-producing Aeromonas strains are more virulent than pigment-producing ones. © 2013.
Other data
Title | Attenuated virulence of pigment-producing mutant of Aeromonas veronii bv. sobria in HeLa cells and Nile tilapia (Oreochromis niloticus) | Authors | Abolghait, Said ; Mohamed, Mohamed F.; Aly, Salah M.; Garbaj, Aboubaker M.; Moawad, Ashraf A. | Keywords | Aeromonas veronii bv. sobria;HeLa cells;Nile tilapia;Pigment-producing aeromonads;Virulence | Issue Date | 1-Jan-2013 | Journal | International Journal of Veterinary Science and Medicine | Volume | 1 | Issue | 1 | Start page | 43 | End page | 47 | ISSN | 23144599 | DOI | 10.1016/j.ijvsm.2013.05.004 | Scopus ID | 2-s2.0-85049733608 |
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