BIOANALYSIS AND BIOTRANSFORMATION OF OLIGONUCLEOTIDE THERAPEUTICS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
Sutton, James Michael; Kim, Jaeah; Morsy EL Zahar, Noha; Bartlett, Michael G.;
Abstract
Since 2016, eight new oligonucleotide therapies have been approved which has led to increased interest in oligonucleotide analysis. There is a particular need for powerful bioanalytical tools to study the metabolism and biotransformation of these molecules. This review provides the background on the biological basis of these molecules as currently used in therapies. The article also reviews the current state of analytical methodology including state of the art sample preparation techniques, liquid chromatography-mass spectrometry methods, and the current limits of detection/quantitation. Finally, the article summarizes the challenges in oligonucleotide bioanalysis and provides future perspectives for this emerging field. © 2020 John Wiley & Sons Ltd.
Other data
| Title | BIOANALYSIS AND BIOTRANSFORMATION OF OLIGONUCLEOTIDE THERAPEUTICS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY | Authors | Sutton, James Michael; Kim, Jaeah; Morsy EL Zahar, Noha ; Bartlett, Michael G. | Keywords | oligonucleotide mass spectrometry;oligonucleotide metabolite identification;oligonucleotide quantitation;oligonucleotide sample preparation;oligonucleotide therapeutics | Issue Date | 1-Jul-2021 | Journal | Mass Spectrometry Reviews | Volume | 40 | Issue | 4 | Start page | 334 | End page | 358 | ISSN | 02777037 | DOI | 10.1002/mas.21641 | PubMed ID | 32588492 | Scopus ID | 2-s2.0-85087183552 |
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