Improved production of Aspergillus awamori ESAWY endochitinase enzyme by optimizing the process parameters using statistical approach

Abstract

The production of endochitinase enzyme by the honey isolate Aspergillus awamori ESAWY has been statistically optimized using multifactorial experiments. The optimization steps included first, 2-level Plackett-Burman design to determine the bioprocess parameters that significantly influence endochitinase production. The second step, central composite design (CCD), aimed to optimize the concentrations of the parameters had the highest positive significant effect on the endochitinase enzyme production. The optimization steps resulted in 10.2 fold increase in activity than the basal medium used first. The new isolate A. awamori ESAWY is suggested as a new potential candidate for endochitinase enzyme production, which has many biotechnological applications. © IDOSI Publications, 2014.