Synergistic interaction between the VA- mycorrhiza Glomus intraradices and Saccharomyces cerevisiae. 11-On the metabolic activities of Sorghum bicolor grown under saline conditions

aboghalia, hoda 


The interactive effects of one of the vesicular arbuscular mycorrhizal (VAM) fungi (Glomus intraradices) and the commercial yeast (Saccharomyces cerevisiae) on some of the metabolic activities of sorghum (Sorghum bicolor) plants grown under saline conditions were studied . The four treatments were: a. non- inoculated plants (Control); b. plants inoculated with Glomus intraradices (VAM); c. plants inoculated with S.cerevisiae (Sc); d. plants inoculated with G.intraradices and S.cerevisiae .Plants were salt-stressed using sodium chloride at concentrations of 0.50 and 100 mM. The contents of total soluble sugars, proline and free amino acids were determined. Extraction of proteins was carried using SDS-Polyacrylamide Gell electrophoresis (SDS-PAGE).The results showed that the treatment of the salinized plants with VAM, S.cerevisiae or in combination statistically increased the accumulation of total soluble sugars, proline and total free amino acids especially when both the VAM fungus and S.cerevisiae were used together. Such increases were related to the increasing of plant tolerance to salinity due to the presence of mycorrhizal fungus and/or yeast strain. SDS-PAGE analysis of proteins revealed that there is an induction of novel proteins as a result of VAM fungus and/or S.cerevisiae treatment, which may play an important role in increasing plant tolerance against salinity through a variety of different mechanisms.

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Keywords mycorrhiza – yeasts - Saccharomyces cerevisiae - Sorghum bicolor – salinity- metabolic activity
Issue Date 2001
Source 2
Journal The African Journal of Mycology and Biotechnology 

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