Chitosan and thidiazuron improve regeneration efficiency of strawberry (Fragaria x ananassa Duch.) cv. Festival from different explant typesYoussef, Sabry Mousa Soliman
AbstractEstablishment of an efficient regeneration system is considered as a prerequisite step for genetic transformation and somaclonal variation which have provided alternative and efficient strategies to the breeders for obtaining genetic variability and improving new genotypes. To optimize the regeneration protocol for strawberry cv. Festival, different explant types, i.e. leaf discs, petioles, stipules, and roots were cultured on N30K mineral formulation with MS microelements medium containing different concentrations of indole-3-butyric acid (IBA) (0.2 or 0.5 mg l–1 ) with thidiazuron (TDZ) (1.0, 1.5 or 2.0 mg l–1 ) or 6-benzylaminopurine (BA) (1.0, 2.0 or 3.0 mg l–1 ) and supplemented with different concentrations of chitosan (0, 25 or 50 mg l–1 ). In addition, the effect of chitosan on shoot multiplication was tested. Results clearly revealed that leaf discs proved to be a good source of explant material for regeneration of Festival cultivar, since the regeneration efficiencies varied from 58 to 100%, from 0 to 40%, from 8.33 to 47.66%, and from 11 to 57.66%, for leaf discs, petioles, stipules, and roots, respectively. In addition, TDZ was more effective than BA and chitosan application acted as a growth stimulator for shoot regeneration from all tested explants. A medium containing 0.5 mg l–1 IBA and 1.5 mg l–1 TDZ supplemented with 25 mg l–1 chitosan was found to be the most efficient combination for the regeneration from leaf discs, petioles and stipules, and produced the highest number of shoots/leaf disc. The medium containing 0.5 mg l–1 IBA and 2.0 mg l– 1 TDZ supplemented with 50 mg l–1 chitosan produced the highest number of shoots regenerated from petioles and stipules, while 0.5 mg l–1 IBA and 2.0 mg l–1 TDZ combination with 25 mg l–1 chitosan gave the highest regeneration frequency and number of shoots for roots. Moreover, chitosan application at 25 or 50 mg l–1 had positive effect on shoot multiplication. Both concentrations accelerated the appearance of the proliferation rate by 5-7 days and increased number of plantlets/explant without significant differences. In conclusion, an efficient regeneration protocol was optimized for strawberry cv. Festival from different explant types.
|Keywords||In vitro, Organogenesis, Adventitious shoot regeneration, Thidiazuron||Issue Date||2016||Journal||Middle East Journal of Agriculture Research, 5 (4): 856-867||URI||http://www.curresweb.com/mejar/mejar/2016/856-867.pdf
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