Effect of Mycotoxins on the Clinicopathological and Immunological Picture in Broilers Infected with Some Pathogenic E. coli Strains with Special References to Prevention and Control

Abstract

Mycotoxins are considered as toxic, active metabolites that constitute a major problem in poultry industry in Egypt as it has a hazard effect on immune response that leads to increase the pathogenicity of other infective agent besides its effect on antibiotics pharmacokinetic. Four hundred and twenty day old chicks were used to study the effect of naturally mycotoxin contaminated diet on the clinicopathological picture and immunological profile in broiler infected with E.coli in addition to its effect on Fluorophenicol absorption, metabolism and excretion. The chicks were allotted into 5 groups. The first 3 groups were further equally subdivided to 2 subgroups A and B each of them was of 30 chicks. The A groups were fed on ration treated with antimycotoxin while the B groups were fed on ration contaminated with mycotoxins. The first 3 groups were inoculated with E. coli O78, O128 and O157 respectively using a dose of 1.5x108/ chick at day old while the groups 4 and 5 were consisted of 120 chicks and kept on mycotoxin treated diet and mycotoxin contaminated diet as –ve and +ve control. On day 17 the control -ve were subdivided into 1A, 2A, 3A and –ve control while the +ve group were subdivided to 1C, 2C, 3C and +ve control. The first 3 subgroups of group 4 and 5 were inoculated with E. coli O78, O128 and O157 respectively then these subgroups furtherly subdivided to Fluorophenicol treated and untreated groups. The results showed high mortality, sever clinical signs, bad feed conversion rate, depraved cellular and humeral immune response and sever histopathological changes in all groups fed on ration contaminated with mycotoxin. Mycotoxicated groups showed lower rate of FF in serum and higher rate of FF in liver and kidney than the free groups were measured through HPLC.