Effect of standard and low protein diets containing Gum Arabic on hyperuricemic rats

Abstract

Effect of standard and low protein diets containing Gum Arabic on hyperuricemic rats. This study was carried out in the Agricultural Research Center, Giza, Egypt. Forty-nine (49) Normal female albino rats (Sprague – Dawely Strain) weighing 200+10 g. were obtained from the Agricultural Research Center, Giza, Egypt. Rats were treated with 30% fructose in drinking water for 6 weeks to induce hyperuricemia. The rats were divided into two main groups as the following: The first main group: (7 rats):fed on standard diet only "as a negative control group"and the second main group: (42 rats with hyperuricemia): The rats in the second main group was divided after confirmation of hyperuricemia into six subgroups as a following: - The first subgroup (7 rats): fed on a standard diet, as a positive control group (1). - The second subgroup (7 rats): fed on a diet containing 5% gum Arabic. - The third subgroup (7 rats): fed on a diet containing 10% gum Arabic. - The fourth subgroup (7 rats): fed on a low protein diet* as a control positive group (2). - The fifth subgroup (7 rats): fed on a low protein diet* containing 5% gum Arabic. -The sixth subgroup (7 rats):fed on a low protein diet* containing 10% gum Arabic.

* Low protein diet: diet containing 7% protein.

At the end of the experimental period (6 weeks), rats were anesthetized with light ether and kidney of each rat were removed and washed in saline (0.9% NaCl), dried with filter paper then weighted immediately absolute weight (g) at the end of each experimental period. Then relative weights (g / body weight) of kidney was determined. According to the method described. The kidneys were kept in 10% formalin for the histological study.