Physiological and genetic studies in buffaloe in respect to reproductive performance

Abstract

The present work was planned to study the effect of environmental temperature in hot and cold seasons of Egypt on the reproductive performances of female buffaloes, as well as their effect on reproductive status and on some blood biochemical, hormonal and antioxidant parameters. Also, identify the expression of some genes that be related to fertility in endometrium and corpus luteum of different reproductive status in Egyptian buffaloes. The present study was carried out on adult female buffalo from slaughterhouse (El wraqe. Giza) of 3-5 years old, the study last from April to September 2017 (hot season), and from October to March 2018 (cold season). The temperature was recorded during the whole study, the mean of temperatures in the hot season was 35℃ and in cold season was 20℃. First experiment: gentialia of female buffaloes were examined for 130 animals in hot season and 190 animals in cold season. Also, identify the reproductive status of each animals and dividing into cyclic, smooth inactive and early pregnant buffalo depending on ovaries macroscopic observation such as (presence, size, and shape of corpus luteum and follicle), the uterus (consistency, color, size and mucus), and identify the percentage of each group in hot and cold months.