BIODIVERISTY OF THERMOPHILIC BACTERIA IN EGYPTIAN HOT SPRINGS

Abstract

The hot springs produced by the immergence of geothermal heated groundwater from the earth’s crust are densely accommodated by numerous microbiota that having the capability to withstand the stressed-environments. Such creatures are producing a variety of exoenzymes of biotechnological applications. Copying with the on-going investigations in this area, geothermal water samples collected from Dakhla Oasis, Kharga Oasis, Pharaoh Baths and Ras Sedr hot springs in Egypt were explored for the isolation of industrially efficient thermostable amylase-, protease- and lipase- producers. The pioneer enzyme producing members were subjected to identification based on morphological and biochemical characteristics besides 16 S rRNA gene sequencing. In addition, statistical multifactorial designs were implemented for monitoring the proper cultivation conditions required for optimization of the enzyme production. The experimental scenario and obtained results are briefly presented in the following: