HISTOLOGICAL CYTOLOGICAL AND CYTOCHEMICAL STUDIES OF THE EFFECT OF SOME CYANINE DYES ON SOME ORGANS IN MICE

Abstract

Summary - Cyanine dyes were used in this study because of their special properties as a class of organic compounds, not only for the chemist, but also for numerous other scientists working in the fields of biology, medical science, technology and physics. - Owing to the widespread use of dyes as food additives, the present investigation was designed to study the effect of 3 types of monocyanine dyes on the liver and kidney tissues of adult male albino Swiss mice (Mus musculus). - Monocyanine dyes were chosen for their structural similarity to the nucleus of natural purine bases, adenine and guanine, and thus may have mutagenic potency, thus producing chromosomal aberrations. - The aim of the work was an attempt to examine whether the dyes employed in this work were toxic to mice, and whether difference in the molecular structure of each of the 3 monocyanine dyes produces different effects on these organs and which one has the least toxic effects. - The work was also aimed to determine whether the injection with these monocyanine dyes produces acute toxic effects or induces tumors. - Animals were divided into four groups: In the 1 group animal s were injected with monocyanine dye type I, in the 21 group animals were injected with monocyanine dye type II, in the 3 group animals were injected with monocyanine dye type III, to find out which of these compounds was more effective, and the 4" group of anima ls were kept without treatment and were used as controls. - In each treated group, anima ls were given ip daily doses of one of the 3 types of monocyanine dyes, up to 28 days according to a planned dose-time schedule. - Material (liver and kidney) was taken from each treated group after 7, 14, 21, 28 days respectively.