“Study of the organic extract of some marine algae and expected biological activity”

Abstract

In this thesis, eco-friendly tools based on marine algae extract is used for synthesis gold nanoparticles. These green routes make applications of the synthesized nanoparticles in biomedical are possible. Taking the above into consideration, the current thesis was dealt with three-fold objectives, representing the four experimental chapters. In the first chapter, presents a general introduction which deals with an overview of marine algae and presenting adequate presentation on the chemical constituents of red and brown algae under study as well as their uses. Also, it deals with the scientific background of nanoscience. In the second, experimental, the algae profiles embraced algae description, family and genus description, scientific classification and taxonomy of the red algae D. simplex, L. papillosa and G. oblongata and the brown alga T. decurrens , the used material and scientific devices used. In the third, Results and Discussion has dealt with chemical analysis, including qualitative and quantitative phytochemical screening, of the algal constituents. The results obtained indicated the presence of carbohydrates, protein, terpenes, phenolics and flavonoids. These algae constituents are responsible for the synthesis of metal nanoparticles, evaluation of the anticancer activity against four different cancer cell colon (HCT-116), Liver (HepG2), and lung (A549) ) and human breast cancer (MCF7) in addition to normal lung cells (WI-38) and The antioxidant activity was measured with method (DPPH/ABTS) radicals for different extracts and gold NPs. Key words : Marine algae, Anticancer,Antioxidants ,Nanogold.