Regenerative potential of immature dog teeth treated with bioceramic scaffold and bone morphogenic protein (An Animal study)

Abstract

The aim of our study was to assess the regenerative potential of young permanent immature teeth with necrotic pulp following different treatment protocols, via either revascularization protocol as a well-established protocol in the literature compared to regeneration with the use of a nano scaffold with or without growth factor (BMP-2). This study was an animal study which was performed for histopathologic and radiographic evaluation of the treatment outcomes over 3 months of follow up. The total number of roots (96) was divided into 2 equal groups according to the post treatment evaluation period; group I and group II for: Evaluation periods of one month and three months respectively. Each group included 48 roots and was further subdivided into 36 experimental roots and 12 control roots. The experimental samples were also subdivided into 3 subgroups according to the treatment protocol: Subgroup (A): Revascularization (12 roots), Subgroup (B): Mesoporous silica Scaffold (12 roots), Subgroup (C): Mesoporous silica Scaffold impregnated with BMP-2 (12 roots) and two control subgroups; subgroup (D) Positive control (6 roots) and subgroup (E) Negative control (6 roots). Each individual root was taken as the unit of measure. Each subgroup was both histopathologically and radiographically evaluated. Induction of infection was done followed by canal disinfection using calcium hydroxide. Treatment protocols were performed according to the previous classification and samples were left for follow up according to the evaluation period for each group.