EXPERIMENTAL CLINICOPATHOLOGICAL AND IMMUNOPATHOLOGICAL STUDIES ON SOME MEDICAL PLANTS IN RELATION TO DIABETES
Khalid Mohammed Mustafa Fararh;
Abstract
The aim of this study was to elucidate the mechanisms underlying the hypoglycemic effect of Nigel/a saliva oil experimentally - induced diabetic hamsters in terms of hepatic glucose production and insulin secretion, and to investigate the possible immunopotentiating effect of
Nigella saliva oil on peritoneal macrophages.
145 Male and 15 female Syrian Hamsters 8 weeks old (80-120gms weight) were used. Diabetes was induced by intraperitoneal injection of
65 mg/kg body weight of Streptozotocin (STZ). Treatment with Nigel/a saliva. oil commenced 6 weeks after induction of diabetes at a dose of
400 mg/kg body weight by gastric gavages. Isolated hepatocytes were
i collected using collagenase to determine liver glucose production.
Phagocytic activity was evaluated by injection of fluorescent latex (2µm diameter) intraperitoneally, followed 24 hrs later by collection of peritoneal macrophages.
Nigella saliva oil (400 mg/kg) reduced blood glucose from 391 mg/dl before treatment to 325, 246, 208 and 179 mg/di after the fist, second, third and fourth weeks of treatment respectively. Hepatic glucose production from gluconeogenic precursors (alanine, glycerol and lactate) was significantly lowered in treated hamsters. High significant increase in serum insulin level was found in STZ+NA diabetic animals treated with Nigella saliva oil. Increase in the relative islets area immunoreactive for insulin (from 18% to 80%) was observed in pancreases of treated hamsters. Treatment with Nigella sativa oil significantly increased the
phagocytic activity and the phagocytic index of peritoneal macrophages'
Nigella saliva oil on peritoneal macrophages.
145 Male and 15 female Syrian Hamsters 8 weeks old (80-120gms weight) were used. Diabetes was induced by intraperitoneal injection of
65 mg/kg body weight of Streptozotocin (STZ). Treatment with Nigel/a saliva. oil commenced 6 weeks after induction of diabetes at a dose of
400 mg/kg body weight by gastric gavages. Isolated hepatocytes were
i collected using collagenase to determine liver glucose production.
Phagocytic activity was evaluated by injection of fluorescent latex (2µm diameter) intraperitoneally, followed 24 hrs later by collection of peritoneal macrophages.
Nigella saliva oil (400 mg/kg) reduced blood glucose from 391 mg/dl before treatment to 325, 246, 208 and 179 mg/di after the fist, second, third and fourth weeks of treatment respectively. Hepatic glucose production from gluconeogenic precursors (alanine, glycerol and lactate) was significantly lowered in treated hamsters. High significant increase in serum insulin level was found in STZ+NA diabetic animals treated with Nigella saliva oil. Increase in the relative islets area immunoreactive for insulin (from 18% to 80%) was observed in pancreases of treated hamsters. Treatment with Nigella sativa oil significantly increased the
phagocytic activity and the phagocytic index of peritoneal macrophages'
Other data
| Title | EXPERIMENTAL CLINICOPATHOLOGICAL AND IMMUNOPATHOLOGICAL STUDIES ON SOME MEDICAL PLANTS IN RELATION TO DIABETES | Other Titles | دراسات اكلينيكية باثولوجية وباثولوجية مناعية على بعض النباتات الطبية التى لها علاقة بمرض السكر | Authors | Khalid Mohammed Mustafa Fararh | Issue Date | 2002 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| B15337.pdf | 940.24 kB | Adobe PDF | View/Open |
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