Possible Protective Effects of Stem Cells Treatment on Ischemic/Reperfused Hearts in Hypertensive Rats Model

Abstract

he present study was planned to assess the possible protective effects of Stem cells treatment on ischemic/reperfused hearts in hypertensive rats. This study was carried out on 49 Adult female rats, weighing 170 -270 grams at the start of the study and will be assigned randomly into 3 groups: 1- Control group (n=17): This group of rats was subjected to oral daily administration of saline for 6 weeks, in addition to IV injection of stem cells buffer in rat tail vein on the third week .After scarification, rats were subjected to ischemia /reperfusion injury on their isolated hearts. 2- Hypertensive untreated group (Ht-u group) (n=16): This group of rats was subjected to daily oral administration of Nitro-L-arginine methyl ester (L-NAME) drug at a dose of (50 mg/kg/d) for 6 weeks to induce hypertension. Then, on the third week they were injected with stem cells buffer at the rat tail. After sacrifice, rats were subjected to ischemia /reperfusion injury of their isolated hearts.