The Effect of Silver Diamine Fluoride on Surface Characterization of Demineralized Dentin and on The Shear Bond Strength of 2 Universal Adhesives

Abstract

This in vitro study was conducted to investigate the effect of Silver Diamine Fluoride (SDF) application on: a- Surface characterization of demineralized dentin using environmental scanning electron microscope and b- The micro shear bond strength of 2 universal adhesives to demineralized dentin. A total of 80 dentin specimens were retrieved from 40 non carious, non-restored human molar teeth for this study. After extraction, the teeth were cleaned from blood and soft tissue was removed using hand scaler. Hard deposits were removed using ultrasonic scaler. The teeth were stored in distilled water at 4⁰C not more than 3 months. Distilled water was changed every 48 hours during the storage period. For ESEM evaluation 40 teeth were used; the roots of each tooth were cut off at the CEJ. Each tooth was sectioned into 2 halves mesio-distally; resulting in 2 halves per each tooth, with total of 80 halves. The 80 halves were divided into 2 groups (n= 40 halves) according to the type of dentin (either demineralized or sound). Each group was subdivided into 2 subgroups (n= 20) according to SDF application (either with SDF or No SDF) The dentin Specimens (n=40 halves) were placed in artificial demineralizing solution for 72 hours. A small cotton pellet or brush was used to paint approximately one drop of SDF onto the middle dentine of each half for at least one minute then air-dry. After the lesions have been exposed to SDF for the determined period of time, the SDF was rinsed off for 30 seconds with distilled water. Each dentin surface was scanned under ESEM to obtain images of dentin surface.