Biocompatibility of a bioceramic based material and MTA when used as a perforation repair material

Abstract

This investigation was conducted to compare MTA and the newly introduced Neo MTA as perforation repair materials in terms of cytocompatibility, biocompatibility, bioactivity, inflammatory tissue reaction, after repair of furcal perforation defect. This study included a cell line part and an animal part to evaluate the various characteristics of the test materials. In the first part (cell line), both materials were mixed according to manufacturer instructions and after setting the materials were sterilized by UV radiation, Periodontal ligament derived mesenchymal stem cells was the cell line of choice, they were isolated by scrapping the root surfaces of freshly extracted teeth, these cells were incubated and after 20 days a cell culture was obtained, PDLSCs from the third passage were seeded on sample discs to conduct the desired tests, for cytocompatibility and viability MTT tests was carried to evaluate the amount of viable mitochondria that reacted and caused the change in the color of formazan and this color change was evaluated by a spectrophotometer, the MTT test was carried on three consecutive days. Evaluation of Alkaline phosphatase activity The relative ALP activity was measured using Alkaline Phosphatase Assay Kit (Colorimetric). The detection was calorimetric, and the assay was enzyme activity and it was read by a microplate reader, the tests was carried on three consecutive days. The ability of materials to upregulate the gene expression of Bone Sialoprotein was evaluated with RT-PCR test to check the bioactivity of the test materials and ability to promote bone deposition.