Remineralizing potential of Grape seed extract on demineralized Enamel and Cementum of Human premolar samples (Scanning electron microscope and polarized light microscopic study)
Abeer Abd El Maksoud Abd El Maksoud Mohamed;
Abstract
Aim:
The aim of this study was to investigate the remineralizing potential of grape seed extract on demineralized enamel and cementum of human premolars and to compare with that of NaF using scanning electron microscope (SEM) attached with energy- dispersive x-ray analysis (EDXA) and Polarized light microscope (PLM).
Materials and Methods:
Forty recently extracted maxillary first premolars were randomly divided into four groups, (n= 10) according to the following procedure: negative GI (no treatment), positive GI (immersed in demineralizing solution), GII (treated with grape seed extract) and GIII (treated with sodium fluoride). The demineralized specimens were subjected to pH cycling twice daily for two weeks. Then analyzed by scanning electron microscope with EDX and finally polarized light microscopy.
Results:
For enamel the SEM of groups GII & GIII revealed apparent decrease in the irregularities of demineralized enamel with presence of globular precipitates causing occlusion of enamel rod ends. EDXA revealed a significant difference between groups, when Ca & P were compared showing a greater potential of remineralization for GSE than NaF. Statistical analysis of PLM showed significant decrease in lesion depth in GII compared with GIII. For cementum the SEM analysis showed regular narrow cracks in some areas with decreaing signs of resorption on the mineralized cementum in both groups II, III. Polarized light microscopic analysis showed mineral precipitation band on the surface of treated cementum lesions without area of demineralization. Wide birefringent zone known as remineralizing zone (RZ) was also observed in GII. While in GIII, thin birefringent zone was also observed.
Conclusion:
In this in vitro study, it was found that grape seed extract caused a better improvement in the surface topography of the enamel and cementum better than sodium fluoride solution.
The aim of this study was to investigate the remineralizing potential of grape seed extract on demineralized enamel and cementum of human premolars and to compare with that of NaF using scanning electron microscope (SEM) attached with energy- dispersive x-ray analysis (EDXA) and Polarized light microscope (PLM).
Materials and Methods:
Forty recently extracted maxillary first premolars were randomly divided into four groups, (n= 10) according to the following procedure: negative GI (no treatment), positive GI (immersed in demineralizing solution), GII (treated with grape seed extract) and GIII (treated with sodium fluoride). The demineralized specimens were subjected to pH cycling twice daily for two weeks. Then analyzed by scanning electron microscope with EDX and finally polarized light microscopy.
Results:
For enamel the SEM of groups GII & GIII revealed apparent decrease in the irregularities of demineralized enamel with presence of globular precipitates causing occlusion of enamel rod ends. EDXA revealed a significant difference between groups, when Ca & P were compared showing a greater potential of remineralization for GSE than NaF. Statistical analysis of PLM showed significant decrease in lesion depth in GII compared with GIII. For cementum the SEM analysis showed regular narrow cracks in some areas with decreaing signs of resorption on the mineralized cementum in both groups II, III. Polarized light microscopic analysis showed mineral precipitation band on the surface of treated cementum lesions without area of demineralization. Wide birefringent zone known as remineralizing zone (RZ) was also observed in GII. While in GIII, thin birefringent zone was also observed.
Conclusion:
In this in vitro study, it was found that grape seed extract caused a better improvement in the surface topography of the enamel and cementum better than sodium fluoride solution.
Other data
| Title | Remineralizing potential of Grape seed extract on demineralized Enamel and Cementum of Human premolar samples (Scanning electron microscope and polarized light microscopic study) | Other Titles | قدرة مستخلص بذور العنب على إعادة التمعدن فى طبقة المينا والملاط المنزوعى المعدن لعينات من الضواحك البشرية (باستخدام المجهر الإلكترونى البيئى الماسح والمجهر الضوئى المستقطب) | Authors | Abeer Abd El Maksoud Abd El Maksoud Mohamed | Issue Date | 2021 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| BB3515.pdf | 1.9 MB | Adobe PDF | View/Open |
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